FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185437
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT3305917.82761800503675No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC2285112.322783479025222No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC137467.412760128776889No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC109955.929237422952269No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC82044.424144048922275No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCGCCTACC41542.2401138931281244No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC37992.048674212805427No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC31541.700847187993766No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCGGGTACC26401.423664101554706No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTGCGGTACC25271.3627269638745234No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT22721.225213954065262No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTCCTTATTCTTCCGGTACC22161.195015018577738No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC18000.9706800692418449No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT17030.9183711988438122No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTACGGGTACC16640.8973397973435722No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGCCTTCCTCCGTGGGTACC15540.8380204597787927No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC13440.7247744517005775No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC12120.6535912466228423No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACC12020.6481985795714987No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC11300.6093713768018248No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTTGGTAAC10380.559758839929464No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTTAGTAGGTACC10150.5473557057113737No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACAGTACC10010.5398059718394926No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT9860.5317169712624773No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC9340.5036751025954906No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT8960.48318296780038505No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC7280.3925861613378129No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC6520.35160189174760165No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC5970.3219422229652119No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC5270.28419355360580684No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC4540.24482708413099868No Hit
TTACCGCGGCTGCTGGCACGTAGCTAGCCGGTGCTTCTTTACCCATTACC4370.23565955014371456No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC4230.22810981627183355No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC4030.2173244821691464No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3040.16393707836084492No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCAGGTACT2670.14398421027087366No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC2480.13373814287332086No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTACTCTTACGGTACC2280.12295280877063369No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTAAC2090.11270674137308087No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCAGGTACT2080.11216747466794652No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC2070.11162820796281217No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCAGGTACT2030.10947114114227474No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1910.10299994068066244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)