FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156901
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG11301272.02758427288545No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG79455.063702589530978No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG25321.6137564451469397No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG13520.8616898553865175No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG12660.8068782225734699No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG9860.628421743647268No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG7250.4620748115053442No Hit
AGAGTTTGATCCTGGCTCAGATCGAACGCTGGCGGCAGGCCTAACACATG5720.3645610926635267No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGGGCCTAACACATG5290.3371552762570028No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGTAGGCCTAACACATG5120.326320418607912No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAACACATG4750.30273866960694956No Hit
AGAGTTTGATCCTGGCTCAGGTTGAACGCTGGCGGCAGGCCTAACACATG4680.29827725763379453No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG4600.29317850109304594No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG4440.2829809880115487No Hit
AGAGTTTGATCCTGGCTCAGATTGAGCGCTGGCGGCAGGCCTAACACATG4370.2785195760383936No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACACG4020.25621251617261837No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACGTG3630.23135607803646888No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCCGGCGGCAGGCCTAACACATG3450.21988387581978447No Hit
AGAGTTTGATCCTGGCTCAGACTGAACGCTGGCGGCAGGCCTAACACATG3350.21351043014384866No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG3340.2128730855762551No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAATACATG3270.20841167360310003No Hit
AGAGTTTGATCCTGGCTCGGATTGAACGCTGGCGGCAGGCCTAACACATG3120.19885150508919636No Hit
AGAGTTTGATCCTGGCTCAGATTGGACGCTGGCGGCAGGCCTAACACATG3070.1956647822512285No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACGCATG3040.19375274854844776No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2960.1886539920076991No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCCAACACATG2770.17654444522342114No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAGCACATG2770.17654444522342114No Hit
AGAGTCTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG2330.1485012842493037No Hit
AGAGTTTGACCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG2240.1427651831409615No Hit
AGAGTTTGATCCCGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG2080.13256767005946424No Hit
GAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGC2010.12810625808630918No Hit
GGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1990.12683156895112205No Hit
AGAGCTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1910.12173281241037341No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1910.12173281241037341No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTCACACATG1900.12109546784277986No Hit
AGAGTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGC1840.11727140043721837No Hit
AGAGTTTGATCCTGGCCCAGATTGAACGCTGGCGGCAGGCCTAACACATG1820.11599671130203121No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1800.11472202216684406No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGTGGCAGGCCTAACACATG1780.1134473330316569No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACCCATG1730.110260610193689No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTAGCGGCAGGCCTAACACATG1670.10643654278812754No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTGACACATG1600.1019751308149725No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCAGCAGGCCTAACACATG1580.10070044167978533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)