FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154813
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2928618.917016012867137No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1770511.436378081944023No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT122717.926336935528671No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC64404.159857376318526No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC62834.058444704256103No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC43502.8098415507741596No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC23451.514730675072507No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC20111.2989865192199623No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC16231.0483615717026349No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC15971.0315671164566282No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC15921.028337413524704No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC15541.0037916712420791No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCCAGGTACC11440.7389560308242847No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC10770.6956780115364989No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC10100.652399992248713No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC9730.628500190552473No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC8520.5503413795999044No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT7630.4928526674116515No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGTACCTACC7520.4857473209614179No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC7510.48510138037503314No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTACGGGTACC7390.47735009333841477No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTTTCTTATGGTACC6220.4017750447313856No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC4910.3171568279149684No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4770.3081136597055803No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC4040.2609599968994852No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3970.2564384127947911No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC3810.2461033634126333No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGGGCTTCCTCTCCGAGTACC3760.24287366048070896No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC3640.2351223734440906No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGTTTCTTTACCGGGTACC3590.2318926705121663No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC3440.2222035617163933No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC3370.21768197761169927No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC3350.21639009643892954No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT3340.21574415585254467No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3330.21509821526615983No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT3280.21186851233423548No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTGATTCCTGAGGTACC3230.20863880940231117No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTCTGGGTACC3180.20540910647038688No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2820.18215524536053174No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTTTCTGCAAGTACC2700.17440395832391337No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2690.1737580177375285No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2660.17182019597837392No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC2620.16923643363283444No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACC2310.14921227545490365No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC2290.14792039428213394No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC2280.14727445369574907No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTATCCAGCTACC2230.14404475076382475No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC2120.13693940431359122No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC2070.1337097013816669No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAAGTACC1950.12595841434504854No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA1880.12143683024035448No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC1870.12079088965396964No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCATACGGTACA1820.1175611867220453No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGCTACC1800.11626930554927559No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1690.10916395909904206No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT1670.10787207792627233No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC1660.10722613733988746No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1590.10270455323519344No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCCTACAGTACC1570.10141267206242369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)