FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59498
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1844531.00104205183368No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC633310.644055262361759No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC53929.062489495445226No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT23133.887525631113651No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC15852.663955090927426No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC9401.5798850381525429No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC7871.3227335372617566No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC5220.877340414803859No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC4690.7882617903122793No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC3990.6706107768328348No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3620.6084238125651282No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC3490.5865743386332314No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3470.5832128811052473No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC3270.5495983058254059No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2890.4857306127937074No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC2260.37984470066220716No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC2050.3445493966183737No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGTTACC2040.34286866785438164No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC2010.33782648156240547No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC1950.3277421089784531No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1810.3042119062825641No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCAGATACC1740.2924468049346196No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC1650.27732024605869104No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC1620.2722780597667149No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC1470.24706712830683386No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC1310.22017546808296076No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA1270.2134525530269925No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1170.19664526538707183No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT1040.17479579145517496No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1040.17479579145517496No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTATTCCGGTACC1020.17143433392719082No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC990.16639214763521465No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC990.16639214763521465No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGTTACT970.16303069010723048No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC940.1579885038152543No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTATTCACGGGATACC900.15126558875928603No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC890.14958485999529397No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCCTTCTGGTAAGATACC890.14958485999529397No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCCCCCACTACC880.1479041312313019No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC850.1428619449393257No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTCTCTGGTAAGATACC840.14118121617533363No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTGCGGTACC830.13950048741134155No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCCGGTAAGATACC780.1310968435913812No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCGGATACC770.12941611482738916No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC750.12605465729940502No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATAAC750.12605465729940502No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC730.12269319977142089No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT730.12269319977142089No Hit
TACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACCG700.11765101347944468No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGTACCTACC700.11765101347944468No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTCCTGGTAAGATACC700.11765101347944468No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCAGGTACC700.11765101347944468No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC680.11428955595146055No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC680.11428955595146055No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC670.11260882718746848No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAGGATACC650.10924736965948435No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC650.10924736965948435No Hit
TTACCGCGGCTGCTGGCACGTAGCTAGCCGTCCCTTTCTGGTAAGATACC630.10588591213150023No Hit
TTACCGCGGCTGCTGGCACGCAGTTAGCCGTCCCTTTCTGGTAAGATACC600.10084372583952401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)