FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183433
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3555919.385279638887223No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2108111.492479542939384No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC78444.276220745449292No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT74554.064154214345293No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC37922.0672398096307645No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC33691.8366379004868265No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC31271.704709621496677No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC28861.5733265006841735No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC21501.1720900819372742No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC20721.129567744080945No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTATCCAGCTACC19661.071780977250549No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC19471.0614229718752897No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC16480.8984206767593617No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC14270.7779407194997628No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC13940.7599504996374697No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGCAGGTACC13060.7119765800046884No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC12920.7043443655176549No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC12730.6939863601423953No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTAGGTACC12340.6727251912142308No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC11320.617119057094416No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC11260.6138481080285444No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC9450.5151744778747553No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC8070.43994264935971167No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTACTTCTTCTGCAGGTACC6910.37670430075286343No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC6550.35707860635763466No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCACCTACC5370.29274994139549587No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGTTACC5150.28075646148730055No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC4810.2622210834473622No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT4240.23114706732158338No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC4190.22842127643335716No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC3950.2153374801698713No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCATGAGTTACC3750.2044343166169664No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTAACAAAGGTAC3620.19734726030757824No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC3560.19407631124170677No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGTTACT3490.19026020399819008No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT3320.18099251497822094No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC3300.17990219862293044No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC3170.17281514231354228No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC3040.16572808600415412No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACTGCTACC2920.15918618787241118No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2800.15264428974066827No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTGCGGGTACC2780.15155397338537777No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTTTCTGCAGGTACC2770.15100881520773252No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGCTACC2720.14828302431950632No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC2580.14065080983247288No Hit
TTACCGCGGCTGCTGGCACATATTTAGCCAGGGCTTCCTCCTGTGATGGT2580.14065080983247288No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC2390.13029280445721325No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCAGGTACC2370.12920248810192278No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC2360.12865732992427753No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGCAGGTACA2340.12756701356898703No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT2280.12429606450311559No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2250.12266058997017985No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2110.11502837548314643No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATCAGGTACC2040.11121226823962974No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCATACGGTACA2030.11066711006198447No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCACTTCTTCTGTTGGTACC2030.11066711006198447No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC2010.109576793706694No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGCTACC1990.1084864773514035No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC1980.10794131917375827No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGTGGGTACC1960.10685100281846778No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC1960.10685100281846778No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTTACCTGTTACC1940.10576068646317728No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTAGTCAGGTACC1930.10521552828553205No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC1930.10521552828553205No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1900.10358005375259631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)