FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162690
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG10002561.48195955498187No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG70624.340770791075051No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG61703.7924887823467945No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG56673.483311820025816No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG43052.64613682463581No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG34452.117524125637716No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG13080.8039830352203576No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAAAGACCAAG11040.6785911856905772No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG6860.4216608273403405No Hit
AGAGTTTGATCCTGGCTCAGTGTGCCAGCAGCCGCGGTAAAGACCAAGTC6160.3786342123056119No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG5970.36695555965332843No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG5860.36019423443358534No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG5450.3349929313418157No Hit
AGAGTTTGATCCTGGCTCAGATCGAACGCTGGCGGCAGGCCTAACACATG4740.2913516503780196No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGGGCCTAACACATG4690.2882783207326818No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG4500.2765996680803983No Hit
AGAGTTTGATCCTGGCTCAGGTTGAACGCTGGCGGCAGGCCTAACACATG4350.267379679144385No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACGTG4160.2557010264921015No Hit
AGAGTTTGATCCTGGCTCAGATTGAGCGCTGGCGGCAGGCCTAACACATG3890.23910504640727764No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACACG3670.2255823959677915No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAATACATG3420.21021574774110272No Hit
AGAGTTTGATCCTGGCTCAGATTGGACGCTGGCGGCAGGCCTAACACATG3360.2065277521666974No Hit
AGAGTTTGATCCTGGCTCGGATTGAACGCTGGCGGCAGGCCTAACACATG3160.19423443358534637No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCCGGCGGCAGGCCTAACACATG2980.18317044686213044No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACGCATG2880.1770237875714549No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAACACATG2870.17640912164238737No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAGCACATG2870.17640912164238737No Hit
AGAGTTTGATCCTGGCTCAGACTGAACGCTGGCGGCAGGCCTAACACATG2860.1757944557133198No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTATGCCTAACACATG2660.16350113713196876No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG2610.16042780748663102No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCTTAACACATG2540.15612514598315816No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG2200.1352265043948614No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCCAACACATG2180.1339971725367263No Hit
GAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGC1950.1198598561681726No Hit
AGAGTTTGATCCCGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1940.11924519023910506No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG1940.11924519023910506No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTGACACATG1930.1186305243100375No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG1880.11555719466469973No Hit
AGAGTCTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1860.11432786280656464No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTCACACATG1850.11371319687749708No Hit
AGAGTTTGACCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1790.11002520130309176No Hit
AGAGTTTGATCCTGGCCCAGATTGAACGCTGGCGGCAGGCCTAACACATG1760.10818120351588911No Hit
AGAGCTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1740.10695187165775401No Hit
GGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1650.10141987829614604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)