FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162690
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC7981949.062019792242914No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT55063.3843506054459405No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC46702.8704898887454666No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC44052.7076034175425656No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC27191.6712766611346734No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC26631.6368553691068906No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC25371.559407462044379No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC22501.3829983404019914No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC18211.1193066568320118No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC13070.8033683692912902No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC11220.6896551724137931No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC10290.6324912410105108No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTGCTGATTAGGTACC7170.44071547114143467No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTGTCGGTAAC7170.44071547114143467No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTACTCTGTCGGTAAC6440.3958448583195034No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC5900.3626528981498556No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTAAC5640.3466715839940992No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTAGGTAAC5550.3411395906324913No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC5530.33991025877435616No Hit
TTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA5450.3349929313418157No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC5420.33314893355461306No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC4710.2895076525908169No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4660.2864343229454791No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT3580.22005040260618353No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3570.219435736677116No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATCCTGTCGGTAAC3530.21697707296084578No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTTTCTGCAGGTACC3510.2157477411027107No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC3510.2157477411027107No Hit
TTACCGCGGCTGCTGGCATAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3430.21083041367017025No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGCGCTTATTCTGTCGGTAAC3380.20775708402483248No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCCGTCGGTAAC3350.20591308623762986No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCCGGTAAC3260.20038109287602188No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT3180.19546376544348149No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTCTCGGTAAC3080.18931710615280595No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCCTATTCTGTCGGTAAC2990.18378511279119797No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2940.18071178314586023No Hit
TACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAACG2850.17517978978425225No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTCAC2540.15612514598315816No Hit
TTACCGCGGCTGCTGGCACAGGGTTAGCCGGTGCTTATTCTGTCGGTAAC2510.1542811481959555No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC2340.1438318274018071No Hit
TTACCGCGGCTGCTGGCACAGAGCTAGCCGGTGCTTATTCTGTCGGTAAC2310.14198782961460446No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCTTTAAGTACC2280.1401438318274018No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTACC2270.13952916589833425No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTCATTCTGTCGGTAAC2260.13891449996926672No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAGC2210.13584117032392895No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCAGGTACC2150.13215317474952365No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCAGTAAC2130.13092384289138853No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC2120.13030917696232097No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA2000.12293318581351036No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGTTACT1960.12047452209724015No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATGCTTCTTCTGTCGGTACC1820.11186919909029443No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT1770.10879586944495666No Hit
TTACCGCGGCTGCCGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1770.10879586944495666No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC1760.10818120351588911No Hit
TTACCGCGGCTGCTGGCACAGAGTCAGCCGGTGCTTATTCTGTCGGTAAC1690.10387854201241624No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGCAAC1680.1032638760833487No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1670.10264921015428115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)