FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143363
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2130414.860180102257905No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1671711.660609780766308No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC115778.075305343777684No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT48463.380230603433243No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC36402.5390093678285193No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC31892.2244233170343812No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC31122.1707135034841625No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC28111.9607569596060348No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC24141.683837531301661No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC20351.4194736438272078No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT16231.1320912648312327No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC13110.914461890445931No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC12340.8607520768957122No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCATAAGGTACC10860.7575176300719154No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC10570.73728925873482No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC9540.6654436639858261No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACAGGTACC9210.6424251724643039No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC9090.6340548119110231No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC8820.6152215006661412No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC6990.4875735022286085No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT6780.47292537126036704No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT6700.4673451308915132No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCCTACAGTACC6150.42898097835564264No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC5510.38433905540481156No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACTGCTACC5460.38085140517427785No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT5460.38085140517427785No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGTCCTTATTCGTATGGTACT4520.3152835808402447No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC4390.30621569024085715No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCGTCAGGTACC4220.2943576794570426No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC4180.2915675592726157No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACC4130.288079909042082No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT3670.2559935269211721No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC3260.22739479503079596No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC3200.22320961475415552No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC3190.22251208470804879No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC3090.21553678424698144No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3010.20995654387812754No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCCAGGTACC2970.2071664236937006No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGTTACC2890.2015861833248467No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCCTCCGGAGGTACT2680.18693805235660527No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCCTCCTCAAGTACC2620.18275287207996485No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC2570.17926522184943117No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGTGGGTACC2520.17577757161889748No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGAAGGTACC2520.17577757161889748No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2280.15903685051233582No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCAGGGCTTGCTTCTTGGTTACA2190.15275908009737518No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC2180.15206155005126845No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC2140.1492714298668415No Hit
TTACCGCGGCTGCTGGCACGAACTTAGCCCGTGCTTCCTCCGGGGATCAC2060.14369118949798762No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC2020.1409010693135607No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCGGGTACC2000.1395060092213472No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCCTCTGTTACC1900.13253070876027984No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTCCTCTGGTACC1850.12904305852974618No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1820.12695046839142596No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC1820.12695046839142596No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCAGGTACT1800.1255554082992125No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATCTGGTACC1700.11858010783814513No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1670.11648751769982493No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC1550.10811715714654409No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCGTAGGGTACA1550.10811715714654409No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGGTACT1540.10741962710043736No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCAGGTACT1520.10602456700822388No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC1480.10323444682379694No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1460.10183938673158346No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)