FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149558
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG4414229.514970780566735No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG2637017.631955495526817No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2349015.706281175196246No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG116047.758862782331938No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG78455.245456612150471No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG22411.498415330507228No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG12040.805038847804865No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG9180.6138086896053705No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG7490.5008090506693055No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTTAGGCATG6560.43862581740863077No Hit
AGAGTTTGATCCTGGCTCAGGATGAATGCTGGCGGCGTGCTTAACACATG5620.3757739472311745No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG5110.3416734644753206No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG4560.3048984340523409No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATG3910.2614370344615467No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG3190.21329517645328233No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG3140.2099519918693751No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG2850.19056152128271306No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG2470.165153318445018No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTTAAGCATG2220.14843739552548174No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2150.14375693710801163No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG2090.13974511560732292No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2040.13640193102341566No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAACACATG1920.12837828802203827No Hit
AGAGTTTGATCCTGGCTCAGGATGAGCGCTGGCGGCGTGCCTAATACATG1850.12369782960456814No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACACG1740.11634282351997219No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAACACATG1690.11299963893606493No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACGTG1620.1083191805185948No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGCGGCGTGCCTAATACATG1600.1069819066850319No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCGTGCCTAATACATG1560.10430735901790611No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAACACATG1500.1002955375172174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)