FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149558
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2730718.25846828655104No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2094114.001925674320331No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1733911.59349550007355No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC96336.440979419355701No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC80785.401249013760548No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC32472.1710640687893656No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC19481.3025047138902635No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC19171.2817769694700383No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT14570.9742039877505717No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC12660.8464943366453148No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC10880.7274769654582168No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC9920.6632878214471978No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC8160.5456077240936626No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC7100.47473221091482903No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC6330.4232471683226574No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT5980.3998448762353067No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCCTCCTCAAGTACC5960.39850760240174377No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC5880.3931585070674922No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC5540.3704248518969229No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT5520.36908757806336007No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC5020.3356557322242876No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCAGGTGCTTATTCTTGGTCCATT4750.31760253547118844No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCATAAGTTACC4580.3062357078859038No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACC4130.2761470466307386No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT3830.2560879391272951No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC3520.23536019470707017No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTCCTTCTTCTGCAGGTACC3350.22399336712178552No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGGATACC3140.2099519918693751No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC2800.1872183366988058No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC2740.18320651519811712No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2640.17652014603030264No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC2570.17183968761283247No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT2450.1638160446114551No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2380.15913558619398493No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2370.15846694927720348No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2270.151780580109389No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCGCAGAGTACC2130.1424196632744487No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCCTCTGTTACC2020.13506465718985278No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC1980.13239010952272695No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1970.13172147260594552No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC1900.12704101418847538No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCAGGTACT1820.12169191885422377No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1580.105644632851469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)