FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148894
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3884426.088358160839253No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1534710.30733273335393No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT71094.774537590500624No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC70754.751702553494432No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC50183.3701828146197967No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC31542.118285491692076No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC24241.6280038147944174No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA20001.3432374709524897No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC19431.3049552030303437No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC17451.1719746934060473No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC17431.1706314559350948No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC15311.0282482840141307No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC11420.7669885959138716No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC9700.6514701734119576No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC9630.6467688422636237No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTACTTCTTCTGCAGGTACC8750.5876663935417142No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTGAGGTACC6730.45199940897551283No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC6490.43588055932408293No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC6430.43185084691122544No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC6270.42110494714360547No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC5070.34051069888645613No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC5020.3371526052090749No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGTTACC4870.3270783241769312No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC4860.326406705441455No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC4770.3203621368221688No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT4690.3149891869383588No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3960.26596101924859294No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT3960.26596101924859294No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT3780.25387188201002053No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC3630.24379760097787687No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC3610.2424543635069244No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC3470.23305170121025698No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC3150.21155990167501712No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC2930.19678428949453974No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGAATACC2890.19409781455263475No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCCTCCTCAAGTACC2710.18200867731406237No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC2610.17529248995929989No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCTTTAAGTACC2560.17193439628191867No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC2480.16656144639810871No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2420.16253173398525125No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC2270.1524574529531076No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT2230.1497709780112026No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC2100.14103993445001142No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2100.14103993445001142No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGTTACT2060.13835345950810643No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATAAC1990.13365212835977272No Hit
CTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC1920.128950797211439No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCAGGTACC1910.12827917847596276No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1900.12760755974048651No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1900.12760755974048651No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC1870.1255927035340578No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTCCTGGTAAGATACC1740.1168616599728666No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCCTTCTGGTAAGATACC1730.11619004123739037No Hit
TTACCGCGGCTGCTGGCACATAGTTAGCCGTCCCTTTCTGGTAAGATACC1690.11350356629548537No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTCTCTGGTAAGATACC1680.11283194756000912No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGCTACC1620.10880223514715168No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCATACGGTACA1560.1047725227342942No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCCGGTAAGATACC1550.10410090399881795No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACC1530.10275766652786546No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC1530.10275766652786546No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGTTAAGATACC1520.10208604779238922No Hit
CTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA1510.10141442905691297No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)