FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119807
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2212618.468036091380302No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC111859.335848489654195No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC84927.0880666405134924No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT68075.681637967731435No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC60985.089852846661714No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC39483.2952999407380204No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC22211.8538148856076857No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC19491.6267830761140836No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC17081.4256262154965904No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC16301.360521505421219No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCATACGGTACA14151.1810662148288498No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC9660.8062967940103668No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGCAGGTACC9450.7887686028362283No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC8980.7495388416369662No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCACTTCTTCTGTTGGTACC7150.5967931756909028No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC6650.5550593871810495No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC6550.5467126294790788No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC6020.5024748136586343No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC5740.4791038920931164No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT5720.47743454055272233No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGTAGGTACC5220.43570075204286896No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC4980.4156685335581394No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC4380.3655879873463153No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC3710.30966471074311186No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATATAGTACC3710.30966471074311186No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3000.2504027310591201No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2990.249568055288923No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC2900.2420559733571494No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2640.22035440333202566No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC2520.2103382940896609No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT2220.18529802098374887No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCCCTACC2190.18279399367315766No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2170.18112464213276352No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTCCTTATTCTTCGTCTACA2010.16776982980961044No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGGTTAGATACC1980.16526580249901926No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC1940.161927099418231No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC1910.15942307210763978No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC1830.15274566594606326No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC1820.15191099017586618No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC1770.14773761132488084No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1760.14690293555468378No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1740.14523358401428965No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGGATACC1700.1418948809335014No Hit
CTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC1670.13939085362291018No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCGCTACC1560.13020942015074244No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTATTCCGGTACC1510.12603604129975712No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC1490.12436668975936296No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCATGTGATACA1460.12186266244877178No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC1430.11935863513818058No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTTAGATACC1400.11685460782758937No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1350.11268122897660404No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACC1340.11184655320640698No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCGTAGGGTACA1310.10934252589581578No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGTTGGTACC1290.10767317435542163No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1280.10683849858522457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)