FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002031176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37000660
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA2172730.5872138496988972No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG1700510.45958909922147334No Hit
CTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGC1623270.43871379591607285No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA1594360.4309004217762602No Hit
CTCGTGTTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATT1205200.32572391951927343No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC1125530.30419187117202773No Hit
CTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGT1052190.2843706031189714No Hit
CTGGTTGTTTGCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACAT1051770.2842570916302574No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT1013960.2740383549915056No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCA951300.2571035219371763No Hit
CCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATC940600.25421168162946284No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGG938270.25358196313254955No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTT928410.2509171458022641No Hit
GGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCC842890.22780404457650216No Hit
CTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTG705670.19071821962094732No Hit
GTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAA671230.18141027754640052No Hit
GTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAA652380.17631577382673716No Hit
GTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATGCTGGGGTAGGCCAGCC644290.17412932634174633No Hit
ATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGGC643990.17404824670695063No Hit
CCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAG636850.17211855139881288No Hit
CATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGG607660.16422950293319094No Hit
GTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGA592970.16025930348269463No Hit
CCAAAGATCACCACGCTGCCACTCTGGTCATCGGCGCTGAGGTAGACAGA562240.15195404622512138No Hit
CTCCACACTCCCCTGGGAAGAACAGTCAGGAGAATTAGATACATCTAAAG547230.147897361830843No Hit
CTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAGCCACTGATGCAGCTC543740.14695413541271968No Hit
TCCAGATGTTGTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATGCTGGGG536210.14491903657934752No Hit
GTTGAGGTTGTGCTTCTTCAGGAAGTCCTTCAGCAGGCCACGCTCGGACA515610.13935156832337586No Hit
GCCAGGTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGC504010.13621648911127532No Hit
CTCAGGGTTGAAGCGGTTGTGGTTGGTGCAGGCAAGACTGGAGCAGTAGA491520.13284087364928085No Hit
CTGATATCATAGAAACAATGTCATTGTAAACTTCTTGAGGGGCATCCATT486010.1313517110235331No Hit
CTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCT484850.13103820310232303No Hit
CAGGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTT479450.12957876967600038No Hit
CGCCATCTGAGTTCTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATG479370.12955714844005484No Hit
GTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCA479340.12954904047657528No Hit
GTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGCCTCCA479170.12950309535019106No Hit
CCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATCA478690.12937336793451792No Hit
CCTGGTTCCAGATGTTGTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATG475770.1285841928225064No Hit
CTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCA472790.12777880178353576No Hit
CACCAAAGATCACCACGCTGCCACTCTGGTCATCGGCGCTGAGGTAGACA459390.12415724476266099No Hit
GTGGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCA455460.12309510154683728No Hit
CTGAATTTTTCTTCCCATAAGGAATTCTATTGACTTCAAGAATATAACCA453430.12254646268471969No Hit
GTTGAAGCGGTTGTGGTTGGTGCAGGCAAGACTGGAGCAGTAGACTGAGG449170.12139513187062068No Hit
CTGGGTGGCACGGGGTACTGGACTCCATTGATGGTGAAGACGATGTCGGG443620.11989515862690016No Hit
GCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAG437210.1181627570967653No Hit
CCCCTCGCTCTGCAGGATGTAGGCACTGGGTGGCACGGGGTACTGGACTC436060.11785195183004844No Hit
GCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGG431730.11668170243449713No Hit
CTAAAGTGGGCACAGGACAGAGGCCAGATCTTCCTGGGAGGTGGCTGAAG429760.11614927949933865No Hit
GGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGG427410.11551415569343898No Hit
GTGCTTCTTCAGGAAGTCCTTCAGCAGGCCACGCTCGGACAGGGTGCGCC412940.11160341464179287No Hit
GTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGAC409460.11066289087816271No Hit
GTCAACAATGGCCTGGCAGCCCTCAGCGCAGGCGATGGCCTCTCCGTTCA405820.1096791246426415No Hit
CGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCG405290.10953588395450242No Hit
GCCATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCA402900.10888994953063No Hit
GGTTGGTGCAGGCAAGACTGGAGCAGTAGACTGAGGGCACCCACAGGTTG398730.10776294260696972No Hit
GGACAGGGAACAGGGCCAAGACCTCCACACTCCCCTGGGAAGAACAGTCA393040.10622513220034453No Hit
CGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTT392500.10607918885771227No Hit
GGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAACAATGGCCTGG389980.10539811992542834No Hit
CTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGAC387720.10478732000996739No Hit
CTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGG383830.10373598741211644No Hit
GTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATCACCA383320.10359815203296373No Hit
CCCTCGCTCTGCAGGATGTAGGCACTGGGTGGCACGGGGTACTGGACTCC381710.10316302465956013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)