FastQCFastQC Report
Wed 27 Sep 2023
EGAF00002031177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37000660
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCT2007340.5425146470360258No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGC1890450.5109233186651265No Hit
CTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTG1749520.47283480889259816No Hit
GCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGC1704640.4607052955271609No Hit
CAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGCTGCT1582730.4277572346006801No Hit
TGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTG1038250.28060310275546435No Hit
CTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCTGGGTC921460.24903880092949693No Hit
CGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCC841760.22749864461877167No Hit
TCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCT791670.21396104826238235No Hit
CCTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAAC742200.20059101648457084No Hit
CTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCCTGCCCG683370.18469130010113333No Hit
ACAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGCTGC672120.18165081379629444No Hit
CCTGGATATGGAGTACTTCGGCACTATCGGCATCGGAACTCCTGCCCAGG658590.17799412226700823No Hit
CCAGAGTGGCAGCGTGGTGATCTTTGGTGGCATTGACTCTTCTTACTACA642040.17352122908077855No Hit
CAACCACAACCGCTTCAACCCTGAGGATTCTTCCACCTACCAGTCCACCA631020.17054290382928305No Hit
CGTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGC613290.16575109741285696No Hit
CTTTGGTGGCATTGACTCTTCTTACTACACTGGAAGTCTGAACTGGGTGC607560.16420247638825902No Hit
CCCGTCTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTCTT577010.1559458669115632No Hit
GCAGAACTCAGAGCTGCTCTTCCTCTGTGGCCAGTTGGGGACCAGCATCA575930.15565398022629867No Hit
CTTCCACCTACCAGTCCACCAGCGAGACAGTCTCCATCACCTACGGCACC569570.15393509196862976No Hit
CTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATCACCGTGGACAGCA544720.1472189955530523No Hit
GAGAAACAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTG537620.1453001108628873No Hit
CATGAAGTGGCTGCTGCTGCTGGGTCTGGTGGCGCTCTCTGAGTGCATCA525660.14206773608903192No Hit
CATGAAGTGGCTGCTGCTGCTGGGTCTGGTGGCACTCTCTGAGTGCATCA525460.14201368299916814No Hit
CTGAACTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATCACCGTGGA507640.13719755269230333No Hit
CACCCGTCTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTC503390.1360489245326975No Hit
CAGGCATCCTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACC499100.13488948575511894No Hit
CCCGTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTG491760.13290573735711741No Hit
CTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCTG483920.13078685623445635No Hit
CTGGAACCAGGGCCTGGTTTCTCAGGACCTCTTCTCTGTCTACCTCAGCG482880.13050578016716458No Hit
GTTACCGTCGAGGGTTACTGGCAGATCACCGTGGACAGCATCACCATGAA479930.1297084970916735No Hit
CAGCGAGACAGTCTCCATCACCTACGGCACCGGCAGCATGACAGGCATCC471310.1273788089185436No Hit
CGGAACCTGGCTCCTTCCTGTATTATGCTCCCTTCGATGGCATCCTGGGG465580.12583018789394568No Hit
CATGTACAAGGTCCCCCTCATCAGAAAGAAGTCCTTGAGGCGCACCCTGT460890.1245626429366395No Hit
GAAACAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGC449210.12140594248859346No Hit
CCGTCTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTCTTC447440.12092757264329879No Hit
CTCTGACACCAATCAGATCTTCGGCCTGAGCGAGACGGAACCTGGCTCCT447260.12087892486242137No Hit
CTGGAAGTCTGAACTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATC444800.1202140718570966No Hit
GGCATCTCTGACACCAATCAGATCTTCGGCCTGAGCGAGACGGAACCTGG438140.11841410396463198No Hit
CCAGTGCCTACATCCTGCAGAGCGAGGGGAGCTGCATCAGTGGCTTCCAG434620.11746276958302906No Hit
CTCCCACCCTGGTAGATGAACAGCCCCTGGAGAACTACCTGGATATGGAG432670.11693575195685699No Hit
GTCTGAACTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATCACCGTG428020.11567901761752358No Hit
CTTCGGCCTGAGCGAGACGGAACCTGGCTCCTTCCTGTATTATGCTCCCT426430.11524929555310635No Hit
CTGGGGCTGGCCTACCCCAGCATTTCCTCCTCCGGGGCCACACCCGTCTT412300.11143044475422871No Hit
GTCCTTGAGGCGCACCCTGTCCGAGCGTGGCCTGCTGAAGGACTTCCTGA408260.1103385723389799No Hit
CTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGAT401500.10851157790158339No Hit
CCTGGGGCTGGCCTACCCCAGCATTTCCTCCTCCGGGGCCACACCCGTCT401240.10844130888476045No Hit
CCTGGCTCCTTCCTGTATTATGCTCCCTTCGATGGCATCCTGGGGCTGGC396510.1071629533094815No Hit
CTGGCAGATCACCGTGGACAGCATCACCATGAACGGAGAGGCCATCGCCT391440.10579270748143411No Hit
CAGAGTGGCAGCGTGGTGATCTTTGGTGGCATTGACTCTTCTTACTACAC390390.10550892875964915No Hit
CTGGTTTCTCAGGACCTCTTCTCTGTCTACCTCAGCGCCGATGACCAGAG386700.104511649251662No Hit
GTCGAGGGTTACTGGCAGATCACCGTGGACAGCATCACCATGAACGGAGA385850.10428192361974084No Hit
CTCATCAGAAAGAAGTCCTTGAGGCGCACCCTGTCCGAGCGTGGCCTGCT382610.10340626356394723No Hit
CTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAACC376200.10167386203381236No Hit
CCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCT375000.10134954349462955No Hit
GGCCTACCCCAGCATTTCCTCCTCCGGGGCCACACCCGTCTTTGACAACA374860.1013117063317249No Hit
ATCGGAGCCAGCGAGAACTCAGATGGCGACATGGTGGTCAGCTGCTCAGC374230.10114143909865392No Hit
CACCGTGGACAGCATCACCATGAACGGAGAGGCCATCGCCTGCGCTGAGG370740.10019821268053056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)