FastQCFastQC Report
Mon 18 Sep 2023
EGAF00002031182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39064911
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA918990.2352469201836912No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG715530.18316437480172423No Hit
CTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGC661910.1694385019845559No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA650980.16664059467587164No Hit
CTCGTGTTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATT506940.12976863047249743No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC476720.12203278794107582No Hit
CTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGT424810.10874464810632745No Hit
CTGGTTGTTTGCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACAT402450.10302084138883615No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCA397600.10177931801764505No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT394690.10103440399493038No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGG390850.10005142466598733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)