FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002031194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36703852
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA1570830.4279741537754675No Hit
CTCGTGTTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATT1358210.3700456289982861No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG1280920.34898789369573524No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA1172920.31956318917153437No Hit
CTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGC1129280.3076734289360147No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC826430.2251616533327347No Hit
CTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGT761110.20736515611494946No Hit
CTGGTTGTTTGCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACAT735710.2004449015324059No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT726390.1979056585123545No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCA706680.19253564993668784No Hit
CCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATC704330.19189539016231866No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGG675210.1839616179794971No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTT657610.1791664809459236No Hit
GGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCC579420.1578635397723378No Hit
CTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTG524900.14300951300697268No Hit
ATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGGC511350.13931780239305672No Hit
CTGAATTTTTCTTCCCATAAGGAATTCTATTGACTTCAAGAATATAACCA508870.13864212399287137No Hit
CATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGG483500.13173004293936233No Hit
CTGATATCATAGAAACAATGTCATTGTAAACTTCTTGAGGGGCATCCATT478060.1302479096744396No Hit
GTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAA469100.12780674900280223No Hit
GTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAA468720.12770321763503187No Hit
GTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATGCTGGGGTAGGCCAGCC443630.1208674228525115No Hit
GTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGA428110.11663898383199671No Hit
GTTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATTGCTGC403250.10986585277207417No Hit
CTGGGATTGGTGGAAAAGGCAATAAAACCAATGGTGGTGCCCTGGGAATG400990.10925011358480848No Hit
CCAAAGATCACCACGCTGCCACTCTGGTCATCGGCGCTGAGGTAGACAGA399230.10877059988145113No Hit
CCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAG383160.10439231282863717No Hit
TCCAGATGTTGTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATGCTGGGG370380.10091038945994005No Hit
CTCCACACTCCCCTGGGAAGAACAGTCAGGAGAATTAGATACATCTAAAG367600.10015297576940971No Hit
CTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAGCCACTGATGCAGCTC367260.10006034244035204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)