FastQCFastQC Report
Thu 14 Sep 2023
EGAF00002031195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36703852
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGCTGCT1787540.48701700301101913No Hit
CTCCTCTGTCCACTGCTTTCGTGAAGACAAGATGAAGTTCACAATTGTCT1376240.3749579199480207No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGC1287590.35080514165107246No Hit
GTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCT1278250.34826044961166475No Hit
CTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTG1110690.30260856544430265No Hit
GCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGC1085590.29577004615210417No Hit
ACAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGCTGC740160.20165730833919013No Hit
TGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTG664860.1811417504625945No Hit
GAGAAACAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTG632230.1722516753827364No Hit
CCTCTGTCCACTGCTTTCGTGAAGACAAGATGAAGTTCACAATTGTCTTT619340.16873978240757945No Hit
CTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCTGGGTC609230.16598530312295287No Hit
CGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCC599950.16345695814161412No Hit
CCTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAAC535600.1459247383626111No Hit
GAAACAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGC506590.13802093578624935No Hit
CTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCCTGCCCG504280.13739157405059282No Hit
TCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCT488770.1331658595397562No Hit
CTGCTTTCGTGAAGACAAGATGAAGTTCACAATTGTCTTTGCTGGACTTC481040.13105981355853333No Hit
CATGAAGTGGCTGCTGCTGCTGGGTCTGGTGGCACTCTCTGAGTGCATCA462490.126005848105534No Hit
CCTGGATATGGAGTACTTCGGCACTATCGGCATCGGAACTCCTGCCCAGG461160.1256434883183378No Hit
CAACCACAACCGCTTCAACCCTGAGGATTCTTCCACCTACCAGTCCACCA436870.11902565431007078No Hit
CCAGAGTGGCAGCGTGGTGATCTTTGGTGGCATTGACTCTTCTTACTACA432590.11785956416781541No Hit
CTTTGGTGGCATTGACTCTTCTTACTACACTGGAAGTCTGAACTGGGTGC431630.1175980112387114No Hit
CCCGTCTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTCTT423240.11531214761872949No Hit
CTTCCACCTACCAGTCCACCAGCGAGACAGTCTCCATCACCTACGGCACC395920.10786878717797795No Hit
CTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATCACCGTGGACAGCA394540.10749280484239093No Hit
CGTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGC382330.10416617852534932No Hit
CCCAAATGAAGAATATGAAGTTGTGACTGAAGATGGTTATATTCTTGAAG380030.10353954129937097No Hit
GCAGAACTCAGAGCTGCTCTTCCTCTGTGGCCAGTTGGGGACCAGCATCA378880.1032262226863818No Hit
CACCCGTCTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTC371350.10117466689872223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)