FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002031200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37942102
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA1586940.41825305303327687No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG1282520.3380202815331633No Hit
CTCGTGTTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATT1208680.3185590508401459No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA1199060.31602360881323865No Hit
CTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGC1189970.31362785330132736No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC852200.22460537373496073No Hit
CTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGT786360.20725261873999495No Hit
CTGGTTGTTTGCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACAT785070.20691262703368413No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT743520.19596173137692796No Hit
CCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATC725090.19110433048754125No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCA717910.18921197354853983No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGG685910.1807780707563329No Hit
GGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCC647440.17063893824332663No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTT646610.17042018388965377No Hit
CTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTG537010.14153406682634503No Hit
ATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGGC507180.13367208806723466No Hit
CTGAATTTTTCTTCCCATAAGGAATTCTATTGACTTCAAGAATATAACCA482800.127246508377422No Hit
GTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAA478650.12615273660905765No Hit
GTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAA476300.12553337187275496No Hit
CCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAG469890.12384395571969102No Hit
GTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATGCTGGGGTAGGCCAGCC461500.12163269183135927No Hit
CATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGG461280.12157470874966284No Hit
CTGATATCATAGAAACAATGTCATTGTAAACTTCTTGAGGGGCATCCATT460760.12143765782928947No Hit
CCAAAGATCACCACGCTGCCACTCTGGTCATCGGCGCTGAGGTAGACAGA436390.11501471373409938No Hit
CTCCACACTCCCCTGGGAAGAACAGTCAGGAGAATTAGATACATCTAAAG427320.11262422941143324No Hit
GTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGA422150.1112616269915673No Hit
CTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAGCCACTGATGCAGCTC414870.10934291410634024No Hit
CAGGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTT391690.10323360577123533No Hit
GCCAGGTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGC389850.10274865636068344No Hit
GTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGCCTCCA385250.10153628283430371No Hit
TCCAGATGTTGTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATGCTGGGG383320.1010276130721487No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)