FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002031218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37384831
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA1433430.38342556637476843No Hit
CTCGTGTTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATT1262260.337639616452994No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG1137790.3043453640328079No Hit
CTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGC1046520.27993171883002493No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA1031290.27585787401312584No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC762280.20390088161693176No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT685890.18346746037182837No Hit
CTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGT682610.18259009917685598No Hit
CTGGTTGTTTGCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACAT652530.1745440550473533No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCA641120.17149201503679395No Hit
CCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATC632820.16927186323244311No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGG610940.16341922209037135No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTT590380.15791966533164212No Hit
GGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCC532990.1425685193013177No Hit
CTGAATTTTTCTTCCCATAAGGAATTCTATTGACTTCAAGAATATAACCA479590.12828465106609685No Hit
CTGATATCATAGAAACAATGTCATTGTAAACTTCTTGAGGGGCATCCATT458940.1227610203721397No Hit
CTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTG456980.12223674356050987No Hit
GTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAA442200.11828326841975025No Hit
ATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGGC433750.11602299339001962No Hit
CATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGG429840.11497711464845194No Hit
GTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAA423840.11337218563325858No Hit
GTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATGCTGGGGTAGGCCAGCC390960.10457717462999899No Hit
CTGGGATTGGTGGAAAAGGCAATAAAACCAATGGTGGTGCCCTGGGAATG385210.10303911765710537No Hit
GTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGA385050.10299631955003354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)