FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002031226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39210625
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCA2231870.5692003124153211No Hit
CTCCGTTCATGGTGATGCTGTCCACGGTGATCTGCCAGTAACCCTCGACG1882770.4801683217240225No Hit
GTCGTATCCGAGGATGCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGA1688830.4307072381529241No Hit
CTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGC1669380.4257468479525639No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC1302610.33220842564993547No Hit
GCCGATAGTGCCGAAGTACTCCATATCCAGGTAGTTCTCCAGGGGCTGTT1102700.28122479557517893No Hit
CTGGAAGCCACTGATGCAGCTCCCCTCGCTCTGCAGGATGTAGGCACTGG1099800.28048520012114064No Hit
CTCGACGGTAACAGGCACCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGT1087750.27741205349315395No Hit
CCCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATC1050950.26802684221432327No Hit
GTCAGAGATGCCTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCA1049710.26771060139949315No Hit
CTCGTGTTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATT1047220.26707556944068095No Hit
GCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGAT1042040.26575449894001946No Hit
CTGGTTGTTTGCCCTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACAT992540.25313036963833146No Hit
GGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTTCC883250.22525782233769545No Hit
ATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGGC865000.220603471635558No Hit
CTCCAACCTGGACAGTGTCGTATCCGAGGATGCCTGTCATGCTGCCGGTG830270.2117461784911615No Hit
CATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCAGG754710.19247589141973637No Hit
GTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAA692740.1766715016656837No Hit
GTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAA682750.17412372284297942No Hit
GTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGA660140.16835742863063266No Hit
CCAAAGATCACCACGCTGCCACTCTGGTCATCGGCGCTGAGGTAGACAGA636090.16222388702041857No Hit
GTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATGCTGGGGTAGGCCAGCC631740.16111449383936113No Hit
GTTGAGGTTGTGCTTCTTCAGGAAGTCCTTCAGCAGGCCACGCTCGGACA583250.14874794778201061No Hit
CCAGTTCAGACTTCCAGTGTAGTAAGAAGAGTCAATGCCACCAAAGATCA534180.13623348263385243No Hit
TCCAGATGTTGTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATGCTGGGG532490.13580247700718873No Hit
GCCAGGTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGC524590.1337877169772224No Hit
GTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCA517740.1320407415082009No Hit
GTTCCGTCTCGCTCAGGCCGAAGATCTGATTGGTGTCAGAGATGCCTCCA517160.13189282241739325No Hit
CCAGGATCCAAAGCTCTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAG514390.1311863812423291No Hit
CTCAGGGTTGAAGCGGTTGTGGTTGGTGCAGGCAAGACTGGAGCAGTAGA513020.1308369861485248No Hit
GTGGGTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCA510950.13030906801409056No Hit
CACCAAAGATCACCACGCTGCCACTCTGGTCATCGGCGCTGAGGTAGACA505440.12890383665141783No Hit
GTGCTTCTTCAGGAAGTCCTTCAGCAGGCCACGCTCGGACAGGGTGCGCC502210.12808008033536827No Hit
CTCCAGATTCGGTGGGGAGGTTCATGCCCTGGAAGCCACTGATGCAGCTC501280.12784289972424567No Hit
GCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGG499020.12726652533592617No Hit
GGCGGATGAAGACATCACCCAGGATCCAAAGCTCTCCAGATTCGGTGGGG495200.1262922995999171No Hit
GTTGAAGCGGTTGTGGTTGGTGCAGGCAAGACTGGAGCAGTAGACTGAGG493020.12573632784481245No Hit
CTCCACACTCCCCTGGGAAGAACAGTCAGGAGAATTAGATACATCTAAAG492710.1256572676411049No Hit
CAGGAGAATTAGATACATCTAAAGTGGGCACAGGACAGAGGCCAGATCTT491950.12546344262556386No Hit
CTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCA489280.12478250474201827No Hit
GGTTGGTGCAGGCAAGACTGGAGCAGTAGACTGAGGGCACCCACAGGTTG472180.12042144189234423No Hit
GCCATCGAAGGGAGCATAATACAGGAAGGAGCCAGGTTCCGTCTCGCTCA469900.11983996684572103No Hit
CTGGGTGGCACGGGGTACTGGACTCCATTGATGGTGAAGACGATGTCGGG468010.1193579546360202No Hit
CTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGGAC461180.11761607982530245No Hit
CCTGGTTCCAGATGTTGTCAAAGACGGGTGTGGCCCCGGAGGAGGAAATG456490.11641997545308191No Hit
GTTATGTTTTTATTCTACGTTATTGGTAGGACAGGGAACAGGGCCAAGAC452980.1155248099207804No Hit
GCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAG452200.11532588424693561No Hit
CGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCG448480.11437716180244513No Hit
CGCCATCTGAGTTCTCGCTGGCTCCGATGTCGCTCTGGATGTTGGCAATG447420.11410682691234837No Hit
GTCAAAGAGGACAGTGAAATCCTGGGCAGGAGTTCCGATGCCGATAGTGC432220.11023032660152701No Hit
GGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGGTGTCAACAATGGCCTGG428940.10939381863971818No Hit
CCCCTCGCTCTGCAGGATGTAGGCACTGGGTGGCACGGGGTACTGGACTC428480.1092765034987328No Hit
CTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGCAGAGAGGTGCCGG423890.10810590241803082No Hit
CTACGTTATTGGTAGGACAGGGAACAGGGCCAAGACCTCCACACTCCCCT419670.10702966351594752No Hit
CGGTGATCTGCCAGTAACCCTCGACGGTAACAGGCACCCAGTTCAGACTT418790.10680523455058419No Hit
GTCAACAATGGCCTGGCAGCCCTCAGCGCAGGCGATGGCCTCTCCGTTCA405000.10328833065017452No Hit
GTTGTGGTTGGTGCAGGCAAGACTGGAGCAGTAGACTGAGGGCACCCACA398490.10162806637231618No Hit
CTGAATTTTTCTTCCCATAAGGAATTCTATTGACTTCAAGAATATAACCA396180.10103894033823739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)