FastQCFastQC Report
Fri 15 Sep 2023
EGAF00002031227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39210625
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCT2111700.5385530069974656No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGC2100990.5358216044758277No Hit
CTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTG1968110.5019328307059631No Hit
GCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGC1844730.47046686963036166No Hit
CAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGCTGCT1432370.365301496724421No Hit
TGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTG1044540.26639208122798347No Hit
CTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCTGGGTC1017510.25949854153051627No Hit
CGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCC867400.22121555063200343No Hit
TCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCT859530.21920844158949265No Hit
CCTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAAC808140.20610230007810385No Hit
CTCAGATGGCGACATGGTGGTCAGCTGCTCAGCCATCAGCAGCCTGCCCG735160.18748999792785756No Hit
CCTGGATATGGAGTACTTCGGCACTATCGGCATCGGAACTCCTGCCCAGG700190.17857149688381657No Hit
CAACCACAACCGCTTCAACCCTGAGGATTCTTCCACCTACCAGTCCACCA667160.17014775969523568No Hit
CATGAAGTGGCTGCTGCTGCTGGGTCTGGTGGCACTCTCTGAGTGCATCA649760.16571018697100595No Hit
CCAGAGTGGCAGCGTGGTGATCTTTGGTGGCATTGACTCTTCTTACTACA635800.16214992747501475No Hit
CTTTGGTGGCATTGACTCTTCTTACTACACTGGAAGTCTGAACTGGGTGC632340.16126751358847252No Hit
CGTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGC626850.1598673828841035No Hit
CCCGTCTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTCTT610600.15572309801233722No Hit
CTTCCACCTACCAGTCCACCAGCGAGACAGTCTCCATCACCTACGGCACC609750.15550632003442946No Hit
GCAGAACTCAGAGCTGCTCTTCCTCTGTGGCCAGTTGGGGACCAGCATCA594290.15156351116565983No Hit
ACAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTGGCTGC572740.1460675518434098No Hit
CTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATCACCGTGGACAGCA533350.13602180531424837No Hit
CAGGCATCCTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACC530860.1353867733554362No Hit
CTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTGCTG529740.13510113649042832No Hit
CTGGAACCAGGGCCTGGTTTCTCAGGACCTCTTCTCTGTCTACCTCAGCG525810.1340988571337488No Hit
CACCCGTCTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTC507530.12943685544415579No Hit
CATGTACAAGGTCCCCCTCATCAGAAAGAAGTCCTTGAGGCGCACCCTGT502240.12808773132282383No Hit
CCCGTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTG500760.12771028260834913No Hit
CAGCGAGACAGTCTCCATCACCTACGGCACCGGCAGCATGACAGGCATCC498980.12725632401931875No Hit
GAGAAACAGAATCCTAACTATTTCTGAGGAAACTGCAGGTCCAAAATGTG487490.12432599582383602No Hit
CGGAACCTGGCTCCTTCCTGTATTATGCTCCCTTCGATGGCATCCTGGGG486840.12416022442896536No Hit
CTCTGACACCAATCAGATCTTCGGCCTGAGCGAGACGGAACCTGGCTCCT485370.12378532604364252No Hit
CCGTCTTTGACAACATCTGGAACCAGGGCCTGGTTTCTCAGGACCTCTTC482340.12301257631063009No Hit
CTGAACTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATCACCGTGGA478080.12192613609193936No Hit
GGCATCTCTGACACCAATCAGATCTTCGGCCTGAGCGAGACGGAACCTGG472370.1204698981462295No Hit
GTTACCGTCGAGGGTTACTGGCAGATCACCGTGGACAGCATCACCATGAA465160.11863111082774119No Hit
CTGGAAGTCTGAACTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATC463650.11824601112581093No Hit
CTTCGGCCTGAGCGAGACGGAACCTGGCTCCTTCCTGTATTATGCTCCCT459430.11716977222372763No Hit
CTCCCACCCTGGTAGATGAACAGCCCCTGGAGAACTACCTGGATATGGAG432910.1104062993130051No Hit
CCAGTGCCTACATCCTGCAGAGCGAGGGGAGCTGCATCAGTGGCTTCCAG431820.11012831343545278No Hit
CTCGGATACGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGAT430340.10975086472097804No Hit
CCTGGCTCCTTCCTGTATTATGCTCCCTTCGATGGCATCCTGGGGCTGGC427080.10891945741747294No Hit
CTCAGTCTACTGCTCCAGTCTTGCCTGCACCAACCACAACCGCTTCAACC426730.10883019589715798No Hit
CCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCT421900.10759838691681144No Hit
GTCCTTGAGGCGCACCCTGTCCGAGCGTGGCCTGCTGAAGGACTTCCTGA418930.10684093915871017No Hit
GTCTGAACTGGGTGCCTGTTACCGTCGAGGGTTACTGGCAGATCACCGTG410590.10471396464606213No Hit
CTGGGGCTGGCCTACCCCAGCATTTCCTCCTCCGGGGCCACACCCGTCTT405520.10342094776607105No Hit
CAGAGTGGCAGCGTGGTGATCTTTGGTGGCATTGACTCTTCTTACTACAC398940.1017428311841497No Hit
CTGGTTTCTCAGGACCTCTTCTCTGTCTACCTCAGCGCCGATGACCAGAG397590.10139853674864911No Hit
CTCATCAGAAAGAAGTCCTTGAGGCGCACCCTGTCCGAGCGTGGCCTGCT397310.10132712753239716No Hit
CATGAAGTGGCTGCTGCTGCTGGGTCTGGTGGCGCTCTCTGAGTGCATCA396480.10111545021279308No Hit
CTGGATATGGAGTACTTCGGCACTATCGGCATCGGAACTCCTGCCCAGGA395770.10093437684301129No Hit
GTACAAGGTCCCCCTCATCAGAAAGAAGTCCTTGAGGCGCACCCTGTCCG395230.10079665906881108No Hit
CCTGGGGCTGGCCTACCCCAGCATTTCCTCCTCCGGGGCCACACCCGTCT393230.10028659323843983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)