FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002031378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41834439
Sequences flagged as poor quality0
Sequence length35-151
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT7857361.878203745005401No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA7727761.847224484114631No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT7674111.8344001218708823No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT6918011.6536638629240374No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA4460861.0663128529104933No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG4386711.0485882217758435No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC4202161.00447384988239No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT3892280.9304009072525151No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA3609290.8627556831824612No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG3350630.800926241654633No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG2972280.7104864009291483No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2954560.7062506563073548No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT2884790.6895730094528099No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2722660.6508178584634541No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG2523150.603127485467177No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2475230.591672808137812No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC2250940.5380590857212164No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG2014680.4815840843473484No Hit
CCCCCACCGAGAACCGCCTCGCGAGCCCCGGGGCCCCGCCACCGGGGGCC1717940.41065209455778773No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT1712120.4092608962677855No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1582340.3782386086257784No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1514230.36195776403264307No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT1355120.3239245063140443No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1311840.3135789630165711No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT1305590.31208497859861345No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC1301300.3110595076941273No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT1244420.2974630543031783No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA1155920.27630823494489787No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA1095980.26198032678291683No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG1059530.25326740965738775No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC991940.2371108645678265No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT950180.22712865828080067No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC941570.22507054534662221No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG940440.22480043296385543No Hit
GTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT934920.22348094592591522No Hit
CCCACCGAGAACCGCCTCGCGAGCCCCGGGGCCCCGCCACCGGGGGCCCC924990.2211073034826641No Hit
CCACCGAGAACCGCCTCGCGAGCCCCGGGGCCCCGCCACCGGGGGCCCCG889370.21259278748783986No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT870060.20797697323011788No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA854720.20431013787468263No Hit
CGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA854130.2041691057456274No Hit
CGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA853660.204056758117397No Hit
CACCGAGAACCGCCTCGCGAGCCCCGGGGCCCCGCCACCGGGGGCCCCGG820660.19616852039058058No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG798280.19081886098675782No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT788180.18840458216733824No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA752870.1799641677996447No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG729980.17449259926731658No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC721350.17242970558300064No Hit
GGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG700360.16741230831373166No Hit
CACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTC661140.1580372572941638No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC644780.15412660368171782No Hit
CCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGG627970.15010838319117892No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT623560.149054227785868No Hit
CAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTT614470.1468813768483904No Hit
GGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG605460.14472764891146264No Hit
AGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGGGAAGAGG588000.14055405404145613No Hit
CCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGG584980.139832160770699No Hit
GCCCCCACCGAGAACCGCCTCGCGAGCCCCGGGGCCCCGCCACCGGGGGC569190.13605775853717078No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT563600.13472153887374946No Hit
GCCCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT559700.1337892925969439No Hit
CCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA559580.1337606080961191No Hit
ACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA553950.13241482693242282No Hit
GGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC551610.13185547916633947No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG550420.13157102453316033No Hit
TGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA545190.13032085837221338No Hit
CGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC542380.1296491629778996No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA527220.12602535437370152No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG520120.12432818807490165No Hit
CGCGTCGGGTGGCGGGGGGGAGAGGGGGGCGCGCCCGGCTGAGAGAGACG513860.12283181328187524No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA512330.12246608589635921No Hit
AGCCGGTGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGG508120.12145973799242295No Hit
GCTCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT497700.11896896717080394No Hit
GGCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGC497670.11896179604559773No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG484680.11585669883131455No Hit
CCCGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA477870.11422885340950789No Hit
GTCCGGGAGGGACCACCGGGCCGCGCTCGGGCGCACGCGCGCGCCGAACG471610.1127324786164815No Hit
GGCCCGCGGGGGGAGGGGGAAGGGGCGGGCGCGGCGGCAAGAGGAGGGCG469480.11222332872684154No Hit
CGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG468190.11191497034297507No Hit
GCCGGTGAGCCGCTCGGGGGGAAGAAGAGGATCGGCGGGCGGCGGGCGGG466670.11155163333252778No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG460810.11015087354225067No Hit
CGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC457380.10933097489367552No Hit
CCCGCGGGGGGAGGGGGAAGGGGCGGGCGCGGCGGCAAGAGGAGGGCGGA444660.1062904178062481No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA444260.1061948028034988No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT438820.10489443876610846No Hit
GGGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGA436050.10423230487206962No Hit
GGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAG433350.10358690360351194No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG425320.10166743242331992No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA421020.10063957114376508No Hit
CGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGA419950.10038380101141071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)