FastQCFastQC Report
Sun 14 May 2023
EGAF00002031386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33956057
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT3349560.9864396210667216No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2782550.8194561577040584No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT2457740.723800175032101No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2342040.6897267253379861No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1710020.5035979295240316No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1700160.5006941765941787No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1618600.4766748977951121No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1296830.3819141898601478No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1285090.3784567801850492No Hit
GCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGAT1247410.36736008541863385No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1137610.33502417551013064No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1080170.3181081949532597No Hit
CCCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAA1073010.31599958734902583No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT930150.27392756467572194No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG915400.26958371521169255No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC881320.25954721421276916No Hit
CTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAA771520.22721130430426595No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG754380.22216360397792947No Hit
GGCCCATTTGAGTATTTTGTTTTCAATTAGGGAGATAGTTGGTATTAGGA736930.2170246091882812No Hit
GGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCC699800.20608988846967713No Hit
GTTCGCTATCGCTCAAAGAGTAGTATTTAGGGTTGGAGAGTGGTCTCCCC656010.19319380928121305No Hit
GTCAGCTAAACGCATTACTGCACTTACACATCTACCCTATCAAGCACATA645870.19020759683611085No Hit
GTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGG639120.18821973352206353No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC636340.1874010283349448No Hit
GGCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATT631700.1860345563679552No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC596020.1755268581390354No Hit
GTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA594800.17516757025116314No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT587340.1729706131663049No Hit
CCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTG581090.17112999898663148No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA573990.16903906127852242No Hit
CCGGGAACGTATTCACCGCAACATGGCTGATTTGCGATTACTAGCGATTC573520.16890064709221098No Hit
GTGTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTG569210.16763135955390815No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC560800.16515462911373957No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT558160.1643771536842455No Hit
GTCCCGCGGCGCCCCGCCTGGGACGCCGGACGGCCCTCGGCCCCCACCGA532370.15678204333324097No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT525990.15490314437863031No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG523890.15428469801426No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG522640.15391657517832533No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN518670.15274741705139674No Hit
CCCCGGGGTACCTTTTATCCTTTGAGCGATGGCCCTTCCACACAGAACCA518250.15262372777852268No Hit
CCCGGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGT504250.14850075201605417No Hit
CGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAG497230.14643337416944494No Hit
CTCCAATCTACGCCCTTAGACTAGCGCTTCCATCAGCTAGCTCGCTTAAC490990.14459570497245897No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT483500.14238991293953832No Hit
GGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGT479580.1412354797260471No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG468850.1380755133023837No Hit
GTGAGTACAAGACCCGGGAACGTATTCACCGCAACATGGCTGATTTGCGA468560.1379901088044469No Hit
CTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTC456750.13451208425053593No Hit
CCCGCCTACGCGCTCTTTACGCCCAGTGATTCCGAGTAACGCTTGCACCC453730.1336226994789177No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG451720.13303075795873473No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC444690.13096043512943803No Hit
ACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTC441390.12998859084257045No Hit
GTTGTCGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGA437800.12893134205776602No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT430630.12681978947084463No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT428160.1260923787470377No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT427610.12593040469922642No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT427070.12577137563410262No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG419940.12367160297793114No Hit
GGGGAGATAGGTAGGAGTAGCGTGGTAAGGGTGATGAGTGTGGGGAGGAA418500.12324752547093439No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG408010.12015823863177047No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA398880.11746946943810349No Hit
CCCCAAGGCACGCCTCTCAGATCGCTAGAGAAGGCTTTTCTCACCGAGGG385720.11359387222138306No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA382230.11256607326345341No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC378400.11143814489414951No Hit
GTGGGGATCAATAGAGGGGGAAATAGAATGATCAGTACTGCGGCGGGTAG376170.11078141375484203No Hit
GCTTGTAGGACATAATAATAACAATTGAATGTCTGCACAGCCGCTTTCCA371760.10948267638966444No Hit
CGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA368790.10860801653148361No Hit
CCTCATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAA360840.10626675529493898No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT359840.10597225702619123No Hit
GTCTGCACAGCCGCTTTCCACACAGACATCATAACAAAAAATTTCCACCA359080.10574843834194295No Hit
GTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT353840.10420526741370471No Hit
CGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCC351000.10336889233046109No Hit
GGTGAGTACAAGACCCGGGAACGTATTCACCGCAACATGGCTGATTTGCG350900.10333944250358633No Hit
GTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGA348030.10249423247228028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)