FastQCFastQC Report
Mon 18 Sep 2023
EGAF00002031387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33956057
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC4708131.3865361340393556No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA3737391.1006548846351625No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG2770260.8158367739811486No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA2734390.8052731210811668No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT2335720.6878654962795003No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG1695810.49941310912512604No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1556490.4583836103231892No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG1377950.4058038894209654No Hit
GTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGC1286340.37882490302098387No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT1268030.3734326397202125No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1143100.3366409710055558No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA1142410.33643776720011986No Hit
GGACCGGGTCGGAGGATGGACGAGAATCACGAGCGACGGTGGTGGTGGCG864420.2545701934709322No Hit
CTGGTGTTAGGGTTCTTTGTTTTTGGGGTTTGGCAGAGATGTGTTTAAGT815220.24008087864854274No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT778820.22936114166612456No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC771890.2273202686637026No Hit
TGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCG755920.22261713131180103No Hit
GTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCGTGCCTAATACATGCAA661230.19473109024407634No Hit
AGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGT625400.18417921727484438No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC604700.1780831031117659No Hit
CGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCAC595860.17547973841603576No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG585510.17243168133449652No Hit
GTGAAATAGAACCTGAAACCATCTGCTTACAATCATTCAGAGCCCTATGA563350.16590559969904634No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA562790.1657406806685476No Hit
GTCCCGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTGGGGGCGT541870.15957977688634462No Hit
CAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGG539920.1590055052622865No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA527950.15548036098537588No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN514640.1515605890283433No Hit
GGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGC508150.1496492952641704No Hit
CTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTTCATGCCCA479570.14123253474335962No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT474960.13987489772443248No Hit
TGACTTTCCAAAAAACACATAATTTGAATCAACACAACCACCCACAGCCT473130.13933596589262412No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG460080.13549276348546593No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT457230.13465344341953484No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG452950.13339299082929446No Hit
CCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTA449420.1323534119406149No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC440550.1297412122968223No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG440400.1296970375565102No Hit
GATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG436050.12841597008745745No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG430350.12673732995559525No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT410940.12102111855920139No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC406370.11967526147102417No Hit
ACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAG403080.11870636216684403No Hit
AGAGCGTTCATACCAGCGTTGAAGGTATACCGGTAAGGAGTGCTGGAGCG400090.11782581234328826No Hit
ACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGT395610.11650646009929834No Hit
GCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG389240.11463050612737515No Hit
GAGCGTTCATACCAGCGTTGAAGGTATACCGGTAAGGAGTGCTGGAGCGG383030.11280167187845162No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG376800.11096694766415312No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT376600.11090804801040356No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC364470.10733578401049333No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA357350.10523895633700933No Hit
GTGGAAGGTCCCGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTG356020.10484727363957481No Hit
GATCCCGCGGCCGTGTTTTCCTGGTGGCCCGGCCGTGCCTGAGGTTTCTC352560.10382830962970759No Hit
CAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAA345140.10164313247559927No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC341910.10069190306754403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)