FastQCFastQC Report
Mon 3 Apr 2023
EGAF00002031398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57124302
Sequences flagged as poor quality0
Sequence length35-151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT15554132.7228569024790885No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA8672661.518208485068229No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC7882131.3798207985105884No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA7097921.2425394712043922No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT7029981.2306461092513656No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT6739141.1797325768636961No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT5990271.048637758409722No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG5853111.0246269617438826No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA5490580.9611636042397507No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG4419090.7735919469090405No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG4365800.7642631677145044No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC4106680.7189024384052868No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG3907480.6840311151635603No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3782340.6621245017575882No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT3137810.5492951143630604No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2991720.5237210600840252No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC2973360.520507016435842No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2685320.470083643210205No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC2617960.45829181422645654No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT2595050.4542812619399708No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC2442890.427644612620387No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT2222680.3890953450949825No Hit
CCGGGAACGTATTCACCGCAACATGGCTGATTTGCGATTACTAGCGATTC2160980.37829433784591365No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA2006650.3512778151757548No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT1993440.3489653142720239No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT1925040.3369914261709491No Hit
CCCCGGGGTACCTTTTATCCTTTGAGCGATGGCCCTTCCACACAGAACCA1880580.3292083989052505No Hit
GTGAGTACAAGACCCGGGAACGTATTCACCGCAACATGGCTGATTTGCGA1859800.32557071769559653No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC1817150.3181045433167831No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA1781130.31179899581092474No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1770520.3099416427005095No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC1716280.30044655950456955No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA1645490.2880542855473315No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG1628220.28503105385865374No Hit
CCCGTAGGGAGTATCTGGTATTAATCATCGTTTCCAATGGCTATCCCAAA1599060.27992639629977445No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT1587260.2778607255454955No Hit
GCCTGTTTGGGTTTCAAATACAGAGGTGGATTTGCCTTCCCTCCAATCTA1581480.276848896989586No Hit
GTCTATCTTAGCCCCGTTATATTTTCAGCGCATGACCACTAGACCAGTGA1571370.27507907230096224No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG1540550.2696838203817353No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1469510.25724778221360145No Hit
GTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1466840.25678038044123497No Hit
CGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA1456350.2549440341520497No Hit
CTGGTATTAATCATCGTTTCCAATGGCTATCCCAAACTAAGAGGCACATG1452220.254221049388052No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC1448070.25349456348718274No Hit
CGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA1400140.24510408897425126No Hit
GGTGAGTACAAGACCCGGGAACGTATTCACCGCAACATGGCTGATTTGCG1368130.2395005194111606No Hit
GGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG1331210.23303742074607756No Hit
GGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG1262080.22093574114918726No Hit
CCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGG1260690.22069241213660695No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG1227260.21484026185562846No Hit
TGTCTATCTTAGCCCCGTTATATTTTCAGCGCATGACCACTAGACCAGTG1185730.20757015114162797No Hit
GACCAGTTAGCTAACAGAAACATCAAGGGTGGTATCTCAAGGATGGCTCC1169870.20479374960240215No Hit
CGCGTTTCAACTTGGCCATGGATAGATCACTTAGCTTCGGGTCTGCAGCA1164340.20382568525738834No Hit
CGCCCTTAGACTAGCACTTCCATCAGCTAGCTCGCTTAACCCTATGCGTC1143350.20015124211058194No Hit
CCCCCACCGAGAACCGCCTCGCGAGCCCCGGGGCCCCGCCACCGGGGGCC1095600.1917922778294954No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC1085070.18994892926656679No Hit
CACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTC1073130.1878587505541862No Hit
CCCGGGAACGTATTCACCGCAACATGGCTGATTTGCGATTACTAGCGATT1012800.17729757118082598No Hit
GCCCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT997100.17454917873657344No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT989980.17330277401026276No Hit
CTCCAATCTACGCCCTTAGACTAGCACTTCCATCAGCTAGCTCGCTTAAC982730.17203361189428626No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA942310.16495781427666284No Hit
CCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGG935700.16380068854057944No Hit
GCTCCCCACGCTTTCGCGCAATCAGCGTCAGTAATGTTCCAGCAGGTCGC919510.16096651824297128No Hit
TGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA900610.15765794389925325No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT896600.15695596595648556No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT893550.15642204258355752No Hit
CCCGCCTACGCGCTCTTTACGCCCAGTGATTCCGAGTAACGCTTGCACCC893460.15640628746763507No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCAATCAGCGTCAGTAATGTTCCAGC886440.15517738842568263No Hit
GGTGTCTATCTTAGCCCCGTTATATTTTCAGCGCATGACCACTAGACCAG882240.1544421496826342No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA878910.15385921039350292No Hit
CCCCCCATCACGCTCCATAGTCGGTATTGGAATATTAACCAATTTGCCAT875220.15321325064068178No Hit
ATCCTGTTTGCTCCCCACGCTTTCGCGCAATCAGCGTCAGTAATGTTCCA866180.1516307367746918No Hit
GTTCGCTATCGCTCAAAGAGTAGTATTTAGGGTTGGAGAGTGGTCTCCCC839760.14700573496722988No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT828640.14505910286658732No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG822930.1440595282897286No Hit
ACGCGTTTCAACTTGGCCATGGATAGATCACTTAGCTTCGGGTCTGCAGC821450.1438004441612258No Hit
GGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC809730.14174877795443347No Hit
CGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC807630.14138115858290926No Hit
CGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC804490.14083148009405874No Hit
CGGTGTCTATCTTAGCCCCGTTATATTTTCAGCGCATGACCACTAGACCA797470.13960258105210635No Hit
GACCCGGGAACGTATTCACCGCAACATGGCTGATTTGCGATTACTAGCGA774180.1355255071650591No Hit
GTTTCAAATACAGAGGTGGATTTGCCTTCCCTCCAATCTACGCCCTTAGA770700.1349163093493904No Hit
CACGCGTTTCAACTTGGCCATGGATAGATCACTTAGCTTCGGGTCTGCAG765360.13398150580465737No Hit
CCCGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA764950.13390973249878835No Hit
GTTCCTTAACCAGAGTTCTTTCACGCGCCTTAGAATACTCATCTCATCTA764390.13381170066638187No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG758730.1328208789317023No Hit
GCTCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT757160.13254603968727707No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA731040.12797355493289003No Hit
CCCCCATCACGCTCCATAGTCGGTATTGGAATATTAACCAATTTGCCATC723460.12664662405853117No Hit
CCCACGCTTTCGCGCAATCAGCGTCAGTAATGTTCCAGCAGGTCGCCTTC716140.12536520796350387No Hit
CCCTACACCAAGAATTCCACCTACCTCTCCCACACTCTAGAATAGTAGTT715920.1253266954579156No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG706560.12368816340197906No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG702120.12291091101647071No Hit
GCCCCGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT701330.12277261611004016No Hit
AGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA689130.12063692261832801No Hit
CCCCGTTATATTTTCAGCGCATGACCACTAGACCAGTGAGCTGTTACGCT683870.11971612362108162No Hit
CAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTT683760.1196968673682875No Hit
GGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA682130.11941152471324726No Hit
AGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCC678420.11876206382355446No Hit
CCAGAGTTCTTTCACGCGCCTTAGAATACTCATCTCATCTACCTGTGTCG674570.11808809497576005No Hit
CTCCAACAACTAGCATCCATCGTTTAGGGCGTGGACTACCAGGGTATCTA661040.11571957588208256No Hit
CACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGC660130.11556027415442205No Hit
CCCCCGTAGGGAGTATCTGGTATTAATCATCGTTTCCAATGGCTATCCCA646140.11311122891269637No Hit
CCCTTCCGGACCCGCCTTCCTCCTCCCCCACCACCACACCGCACGCAACA636940.11150070595173313No Hit
CCTCCAACAACTAGCATCCATCGTTTAGGGCGTGGACTACCAGGGTATCT631210.11049763023800274No Hit
CCCGGGGTACCTTTTATCCTTTGAGCGATGGCCCTTCCACACAGAACCAC622210.10892211864575606No Hit
CCCCGACCCTCTCTCCCCGCCGGCACCCTTCCCCTTCCGGACCCGCCTTC617500.10809760091248029No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG616610.10794180032169146No Hit
GTCCCGCGGCGCCCCGCCTGGGACGCCGGACGGCCCTCGGCCCCCACCGA604880.10588838354646328No Hit
CATCGAGGTGCCAAACCTCCCCGTCGATGTGAGCTCTTGGGGGAGATCAG593320.10386472643464423No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG587990.10293167345834701No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG583710.10218243016781195No Hit
ACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGG579050.10136666527671534No Hit
CCTGTTAGCAACTAAGAAAGGGGGTTGCGCTCGTTGCGGGACTTAACCCA577060.10101830215798523No Hit
GTCAGCTAAACGCATTGCTGCGCTTACACATCTACCCTATCAAGCACATA576240.1008747555462472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)