FastQCFastQC Report
Mon 18 Sep 2023
EGAF00002031402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43799577
Sequences flagged as poor quality0
Sequence length35-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT9231272.1076162447870215No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA7129091.6276618379214027No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT6532121.4913660010917456No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT5704931.3025080128056945No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA4715091.076514962690165No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT4709961.0753437185021217No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC4399021.004352165318857No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG3769600.8606475811398818No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA3506560.8005922066324979No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG2887580.6592712071169089No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2835660.6474172113580001No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG2743110.6262868703047064No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG2683010.6125652766007307No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT2587070.5906609554699581No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG2540660.5800649627278364No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2289760.5227813044861142No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2272700.5188862897009257No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT2009650.4588286320664695No Hit
CCCCGGGGTACCTTTTATCCTTTGAGCGATGGCCCTTCCACACAGAACCA1934860.4417531246934188No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1886450.4307005065368553No Hit
GTGAGTACAAGACCCGGGAACGTATTCACCGCAACATGGCTGATTTGCGA1779380.4062550649747142No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1713850.391293733270529No Hit
CCGGGAACGTATTCACCGCAACATGGCTGATTTGCGATTACTAGCGATTC1705250.3893302439884294No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1617430.3692798220402905No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT1577030.36005598866856636No Hit
GGTGAGTACAAGACCCGGGAACGTATTCACCGCAACATGGCTGATTTGCG1406120.3210350638774434No Hit
GTCTATCTTAGCCCCGTTATATTTTCAGCGCATGACCACTAGACCAGTGA1357630.30996418070430226No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC1329740.3035965392999115No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC1321140.3016330500178118No Hit
GTTCGCTATCGCTCAAAGAGTAGTATTTAGGGTTGGAGAGTGGTCTCCCC1275010.291100984833712No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA1239220.2829296730422762No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT1167470.26654823629917707No Hit
GTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1115030.2545755179325134No Hit
TGTCTATCTTAGCCCCGTTATATTTTCAGCGCATGACCACTAGACCAGTG1102550.2517261753463966No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT1081410.24689964471574694No Hit
CGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA1033920.23605707424982666No Hit
GTCAGCTAAACGCATTGCTGCGCTTACACATCTACCCTATCAAGCACATA1031120.23541779866960816No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC997410.22772137730919184No Hit
CTGGTATTAATCATCGTTTCCAATGGCTATCCCAAACTAAGAGGCACATG987920.2255546897176655No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA981730.2241414340599682No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA978990.22351585724218298No Hit
CGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA936990.2139267235389054No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA929740.21227145641155393No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC927630.21178971659931786No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC907590.20721432994661115No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG894040.204120692763768No Hit
CCCGGGAACGTATTCACCGCAACATGGCTGATTTGCGATTACTAGCGATT888480.20285127411161985No Hit
GGTGTCTATCTTAGCCCCGTTATATTTTCAGCGCATGACCACTAGACCAG856390.19552471933690138No Hit
CGCCCTTAGACTAGCGCTTCCATCAGCTAGCTCGCTTAACCCTATGCGTC837450.19120047666213763No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT800660.18280085216348094No Hit
GGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG783630.1789126867595091No Hit
CGGTGTCTATCTTAGCCCCGTTATATTTTCAGCGCATGACCACTAGACCA782770.17871633783129914No Hit
CCCCGTTATATTTTCAGCGCATGACCACTAGACCAGTGAGCTGTTACGCT779900.17806108036157517No Hit
CGCGTTTCAACTTGGCCATGGATAGATCACTTAGCTTCGGGTCTGCAGCA763390.17429163756535823No Hit
CCTGTTAGCAACTAAGAAAGGGGGTTGCGCTCGTTGCGGGACTTAACCCA755840.17256787662584047No Hit
GGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG754580.17228020261474214No Hit
CCACCAAGCATTGTCCTGCCTGTGGATAACACAGGCCAGTTAGCTAACAG743250.1696934196419294No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC739140.16875505441525154No Hit
CCCGCCTACGCGCTCTTTACGCCCAGTGATTCCGAGTAACGCTTGCACCC722310.16491255155272388No Hit
GCCTGTTTGGGTTTCAAATACAGAGGTGGATTTGCCTTCCCTCCAATCTA719620.1642983903702997No Hit
GCCCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT709360.16195590199421334No Hit
GACCCGGGAACGTATTCACCGCAACATGGCTGATTTGCGATTACTAGCGA678550.15492158748473758No Hit
CCCCCCATCACGCTCCATAAGTCGGTATTGGAATATTAACCAATTTGCCA669380.15282795995952198No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG659290.15052428474366317No Hit
TGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA656750.1499443704673221No Hit
AAAGGTCCACGGGGTCTCTCCGTCTTGCCGCGGGTAGGAGGAATTTTCAC652600.14899687273235537No Hit
CTGTTAGCAACTAAGAAAGGGGGTTGCGCTCGTTGCGGGACTTAACCCAA642480.14668634813527992No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT640000.14612013262137213No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT631490.14417719148292232No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG629850.14380275864308006No Hit
GTTAGCAACTAAGAAAGGGGGTTGCGCTCGTTGCGGGACTTAACCCAACA627860.14334841635571047No Hit
CGCTCAAAGAGTAGTATTTAGGGTTGGAGAGTGGTCTCCCCGGCTTCAAC619430.14142374023383833No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT613710.14011779154853482No Hit
CCCGGGGTACCTTTTATCCTTTGAGCGATGGCCCTTCCACACAGAACCAC611480.13960865421143223No Hit
CAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTT610210.1393186970732617No Hit
GTTCCTTAACCAGAGTTCTTTCACGCGCCTTAGAATACTCATCTCATCTA608110.1388392403880978No Hit
GCTCCCCACGCTTTCGCGCAATCAGCGTCAGTAATGTTCCAGCAGGTCGC599430.13685748608942044No Hit
CCCGTAGGGAGTATCTGGTATTAATCATCGTTTCCAATGGCTATCCCAAA574020.13105606019893754No Hit
CCAGAGTTCTTTCACGCGCCTTAGAATACTCATCTCATCTACCTGTGTCG573920.13103322892821545No Hit
GTGGTATCTCAAGGATGACTCCATAAGAGCCAAAGCCCTTACTTCAAAGC568540.12980490656336705No Hit
CATCGAGGTGCCAAACCTCCCCGTCGATGTGAGCTCTTGGGGGAGATCAG561770.12825922953548158No Hit
CCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGG556840.1271336478888826No Hit
CACGCGTTTCAACTTGGCCATGGATAGATCACTTAGCTTCGGGTCTGCAG555010.12671583563466834No Hit
CCCACCAAGCATTGTCCTGCCTGTGGATAACACAGGCCAGTTAGCTAACA554640.12663135993299662No Hit
GGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC553880.12645784227550874No Hit
CGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC549590.1254783807615311No Hit
CCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGG542220.1237957161093131No Hit
CGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC542090.12376603545737438No Hit
GCCCCGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT538810.12301716977768987No Hit
ACGCGTTTCAACTTGGCCATGGATAGATCACTTAGCTTCGGGTCTGCAGC538110.12285735088263523No Hit
GGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG533910.12189843751230747No Hit
GCTCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT532740.12163131164485902No Hit
AGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG528380.12063586824137594No Hit
CTCCAATCTACGCCCTTAGACTAGCGCTTCCATCAGCTAGCTCGCTTAAC526120.12011988152305672No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCAATCAGCGTCAGTAATGTTCCAGC524630.11977969558929757No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG518360.11834817491502259No Hit
CCCTACACCAAGAATTCCACCTACCTCTCCCACACTCTAGAATAGTAGTT512320.11696916616340837No Hit
CCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA510880.1166403958650103No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA510340.116517107003111No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG507730.11592121083726448No Hit
CGCTATCGCTCAAAGAGTAGTATTTAGGGTTGGAGAGTGGTCTCCCCGGC503250.11489836990891486No Hit
GTCCTCAACCCCGAATGCAAGCACTCGGTTTGCCCTTTTCCCCGTTCGCT501360.11446685889226738No Hit
CCCACGCTTTCGCGCAATCAGCGTCAGTAATGTTCCAGCAGGTCGCCTTC501160.11442119635082319No Hit
CCCCCATCACGCTCCATAAGTCGGTATTGGAATATTAACCAATTTGCCAT501150.11441891322375099No Hit
CCCCCTTTTATTATCACTTGAGGCTATACCTAAATATATTTCGAAGAGAA500140.11418831738945788No Hit
CCCGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA498720.11386411334520423No Hit
ACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA498460.1138047520413268No Hit
ATCCTGTTTGCTCCCCACGCTTTCGCGCAATCAGCGTCAGTAATGTTCCA495980.11323853652741897No Hit
CCTCCAACAACTAGCATCCATCGTTTAGGGCGTGGACTACCAGGGTATCT491610.11224080999686367No Hit
ACCCACCAAGCATTGTCCTGCCTGTGGATAACACAGGCCAGTTAGCTAAC490490.11198509976477627No Hit
GGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA489970.1118663771570214No Hit
CTCCAACAACTAGCATCCATCGTTTAGGGCGTGGACTACCAGGGTATCTA479490.10947365998534644No Hit
GTTTCAAATACAGAGGTGGATTTGCCTTCCCTCCAATCTACGCCCTTAGA475050.10845995156528566No Hit
CGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG468600.10698733460371089No Hit
CCCCCACCGAGAACCGCCTCGCGAGCCCCGGGGCCCCGCCACCGGGGGCC468050.10686176261473941No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA466960.10661290176386864No Hit
AGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA451670.10312200047046118No Hit
GGCCAGTTAGCTAACAGAAACATCAAGGGTGGTATCTCAAGGATGACTCC450730.10290738652567354No Hit
GTACAAGACCCGGGAACGTATTCACCGCAACATGGCTGATTTGCGATTAC445960.10181833491222986No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG441600.10082289150874678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)