FastQCFastQC Report
Sat 1 Apr 2023
EGAF00002031431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25341248
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT744400.2937503314753875No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT719140.2837823930376278No Hit
GTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCT503790.19880236364049633No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC450260.17767869995984412No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG449630.17743009341923494No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT445510.17580428556636202No Hit
GCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGC430530.16989297448965418No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT429500.16948652252643595No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT414230.1634607735183366No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT412730.16286885318355276No Hit
CTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGCTG406800.16052879479337404No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT389360.15364673436762072No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG377320.14889558714708923No Hit
TGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTG359720.14195038855229228No Hit
CTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGC357990.14126770709950828No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT355510.14028906547933234No Hit
CGTCTTGCCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGC319510.1260829774445205No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA308550.12175801286503332No Hit
GTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAG303800.11988359847155121No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG292040.11524294304684599No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA288720.11393282603919111No Hit
GTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAG285170.11253194791353606No Hit
CGACACTGTCCAGGTTGGAGGCATCTCTGACACCAATCAGATCTTCGGCC276610.10915405586970303No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC275870.10886204183787633No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG273670.10799389201352672No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT267920.10572486406352205No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT267050.10538155026934744No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT259330.10233513361299335No Hit
CATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGC254700.1005080728462939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)