FastQCFastQC Report
Fri 15 Sep 2023
EGAF00002031435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002031435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39164363
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCGTGCCTAATACATGCAA2317530.591744591888294No Hit
ACCTTACCTAGGCTTGACATTGAGAGAATCCGCTAGAAATAGTGGAGTGT1127840.28797608683179654No Hit
GTGAAATAGAACCTGAAACCATCTGCTTACAATCATTCAGAGCCCTATGA1124120.28702624373081215No Hit
CCTTACCTAGGCTTGACATTGAGAGAATCCGCTAGAAATAGTGGAGTGTT1077540.2751327782351522No Hit
CGCCTCCTAAAAAGTAACGGAGGCTTGCAAAGGTTGGCTCATTGCGGTTG918930.23463422601817885No Hit
GTGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGATGAAGCTTT792480.20234722060971602No Hit
AGAGCGTTCATACCAGCGTTGAAGGTATACCGGTAAGGAGTGCTGGAGCG734840.18762975922779593No Hit
GAAGGTTTTAGGATTGTAAACTCCTTTTGTTAGAGAAGATAATGACGGTA723990.18485938351659134No Hit
CTAGGCTTGACATTGAGAGAATCCGCTAGAAATAGTGGAGTGTTTGGCTT719710.1837665532821254No Hit
AGCGGAGTGAAATAGAACCTGAAACCATCTGCTTACAATCATTCAGAGCC692310.17677039710820777No Hit
ATCGCTGATACCGTCGTGCCAAGAAAAGCCTCTAAGCATATCCATAGTCG670990.1713266726692325No Hit
ATGGCGGATGCCTTGCCAAAGAGAGGCGATGAAGGACGTACTAGACTGCG651600.16637574317243461No Hit
CCTGGCTCAGAGTGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAAC600690.15337668073396216No Hit
CTAAAAAGTAACGGAGGCTTGCAAAGGTTGGCTCATTGCGGTTGGAAATC591860.15112208003995878No Hit
AGAAGAGCGTTCATACCAGCGTTGAAGGTATACCGGTAAGGAGTGCTGGA577160.14736866778606866No Hit
CGGAGGCTTGCAAAGGTTGGCTCATTGCGGTTGGAAATCGCAAGTTGAGT577090.14735079439438348No Hit
AAAAAGTAACGGAGGCTTGCAAAGGTTGGCTCATTGCGGTTGGAAATCGC543660.13881497319387015No Hit
CCTCAAGTGATAATAAAAGGGGGTAGAGCTCTGATTGGGCTAGGGCTGCT539090.13764809605099412No Hit
GAGCGTTCATACCAGCGTTGAAGGTATACCGGTAAGGAGTGCTGGAGCGG509180.13001105111807895No Hit
CTTACCTAGGCTTGACATTGAGAGAATCCGCTAGAAATAGTGGAGTGTTT488070.12462094685415923No Hit
GAAGAGCGTTCATACCAGCGTTGAAGGTATACCGGTAAGGAGTGCTGGAG465620.11888869480655155No Hit
CTCCTAAAAAGTAACGGAGGCTTGCAAAGGTTGGCTCATTGCGGTTGGAA452160.11545189691965627No Hit
AGTGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGAACGATGAAGCTT440290.11242108035818174No Hit
CGCTGATACCGTCGTGCCAAGAAAAGCCTCTAAGCATATCCATAGTCGTC437940.1118210450658932No Hit
CAAACCCTATCAAACTTCGAATACCTTTTATCGTATCTTGGGAGTCAGGC421500.10762335136154265No Hit
ATTTAGGTATAGCCTCAAGTGATAATAAAAGGGGGTAGAGCTCTGATTGG419360.10707693624430967No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT414950.10595091256814264No Hit
GGCGGATGCCTTGCCAAAGAGAGGCGATGAAGGACGTACTAGACTGCGAT414670.10587941900140187No Hit
TGAAGGTTTTAGGATTGTAAACTCCTTTTGTTAGAGAAGATAATGACGGT394340.10068847538768853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)