FastQCFastQC Report
Fri 10 Feb 2023
EGAF00002032324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002032324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114778499
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1191282210.378966534490052TruSeq Adapter, Index 2 (100% over 50bp)
GACCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC4471240.38955379613389085TruSeq Adapter, Index 2 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1839520.16026695034581348TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC1150910.10027226440729113TruSeq Adapter, Index 2 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)