FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002035613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002035613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10031768
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG9600.026.6224213
CCCGTAT13000.023.164821
CCGTATC15500.018.7480142
GTATCGA11950.018.4573154
CGGTTCG6350.017.0916443
TATCGAA15650.014.9884385
CGCCCTA7800.013.01250130
ATCGAAG22550.012.7310216
TCGCGAG3851.8699211E-812.72702313
CTCCAAT78100.012.6436351
GTCGTAT5053.6379788E-1112.4811611
GTCCGCT12150.012.3927041
TATGCCG7100.012.32339448
ATCGCGC14000.012.24963867
GTCGGTT19500.012.2109141
GTCCCGT11000.012.0966411
TATACGG8700.012.068727513
GTACGGG7850.012.037559568
CATCGCG15600.011.89073366
CTCGCGA4451.1061275E-811.797461512
Produced by FastQC (version 0.11.5)