FastQCFastQC Report
Sat 23 Apr 2022
EGAF00002035617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002035617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10339875
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8100.029.8168848
CGTATGC9600.025.522646
TCGTATG10750.022.79227645
GCCGTCT12300.019.92075251
TGCCGTC12700.019.5681950
CGTATCG3451.4551915E-1116.2301833
ATGCCGT15600.015.93074349
ACGTCTG15800.015.50567815
CCGAACG2101.5821488E-515.00183735
ACACGTC16100.014.99937113
CGATCGG1400.003759006714.9985718
CGTCTGA17200.014.65054716
CTCGTAT16450.014.25582344
CCCGTAT10300.013.9328011
GTCCGGT5350.013.7390691
CCCGTCT12750.013.7262411
CTCGCGA1800.001259943713.6118551
AATCTCG17200.013.430058541
CTCCTAT44800.013.2819761
CCCGACT8100.012.9636721
Produced by FastQC (version 0.11.5)