FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002041154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002041154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87968534
Sequences flagged as poor quality0
Sequence length18-150
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCAC3208680.36475315139388365No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG2133180.24249352615106667No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG1614300.1835087987256898No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG1554920.17675865781735092No Hit
CTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCC1505380.1711270986964498No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG1414980.1608506969094199No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGG1215180.13813803012790915No Hit
CCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACT1162230.13211883239977604No Hit
GGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGG1128820.12832088346498988No Hit
GTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGCCCGGCGG1011670.11500362163589085No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG969580.11021895624633235No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG934940.10628118458811647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)