FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002044558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002044558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221276
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG158717.172490464397405No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA129895.870044650120212No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA126575.720005784630958No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG119855.416312659303314No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG92664.18753050488982No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG75403.4075091740631605No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC64022.8932193278981906No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA62742.8353730183119725No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG58472.642401344926698No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC48162.1764673981814564No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCACGTCCCAGGG45632.0621305518899473No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA37711.704206511325223No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA30081.3593882752761257No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG21560.9743487770928615No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA18890.8536849906903595No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG18540.8378676404128781No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATATATGTGTAGCC16990.7678193748983171No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT16840.7610405104936822No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA16020.723982718415011No Hit
CCTGTTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC15810.7144923082485222No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA15300.6914441692727634No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA14880.6724633489397857No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA14220.6426363455593919No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG14060.6354055568611146No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA13020.5884054303223124No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG11920.5386937580216562No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA10950.49485710153835033No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG10350.46774164391981055No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA8940.4040203185162422No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAACGGGCCAGTG8820.39859722699253425No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG8770.3963376055243226No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA8700.3931741354688263No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG8680.39227028688154164No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA8140.36786637502485586No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG7750.350241327572805No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT7720.348885554691878No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG7480.3380393716444621No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG7330.3312605072398272No Hit
CCTGTTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT7130.3222220213669806No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG6550.2960104123357255No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA6150.27793344059003233No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5900.26663533324897415No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGACCAGGT5860.2648276360744048No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA5790.2616641660189085No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATTGGGCCAGGT5500.24855836150328098No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA5470.247202588622354No Hit
CCCTTTTGCTCCCCACGCTTTCGCACCTCAATGTCAGTCAAGTGCAAGAA5290.2390679513367921No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGTG5160.23319293551944176No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA5130.23183716263851478No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG4860.21963520671017192No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA4560.20607747790090206No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGTTGTTTCCCAGAG4370.1974909163216978No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA4310.19477937055984382No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA4230.1911639762107052No Hit
CCTGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTATTTCCCAGAG4070.18393318751242793No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA4040.18257741463150093No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTAACGCTCCGGAC3990.18031779316328927No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3670.16585621576673476No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA3550.1604331242430268No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA3390.15320233554474955No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTA3230.1459715468464723No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGCA3220.14551962255282996No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA3210.14506769825918764No Hit
CCTGTTTGCTCCCCACGCTTTCGTCTCTCAGTGTCAGGTCTGTTCCAGGA3170.1432600010846183No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT3040.13738498526726803No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2980.13467343950541405No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2930.1324138180372024No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2870.12970227227534845No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCAGGCCAGAT2850.12879842368806377No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG2840.12834649939442144No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA2830.12789457510077912No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA2570.11614454346607855No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG2510.11343299770422459No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAACGTCGGTGTCGGACCAGGA2480.1120772248232976No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA2440.11026952764872829No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2270.1025868146568087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)