FastQCFastQC Report
Wed 8 May 2024
EGAF00002047690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758315
Total Bases151.7 Mbp
Sequences flagged as poor quality0
Sequence length74-288
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA68931590.90087892234757No Hit
CTTACCTGAGGAGACGGTGACCAGGGTACCCTGGCCCCAGTAGTCGAAAG130811.7250087364749478No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATGGTCAGAGC36830.48568207143469405No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAGTAGTCAAAAA34350.4529779840831317No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGGAGTCAAGGA33040.4357028411675887No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG31260.4122297462136448No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGACCCCAGTAGTCAAAAA30920.4077461213348015No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAT24450.32242537731681425No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA20770.27389673156933464No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG19610.2585996584532813No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC16820.22180756018277364No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAG15180.2001806637083534No Hit
CTTACCTGAGGAGACGGTGACCAGGGTACCCTGGCCCCAGTAGTCAAAAA14990.19767510862899984No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAG12340.16272920883801587No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA11300.14901459156155425No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT11010.1451903232825409No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGTAGTCAAAAA9710.12804705168696387No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAGTAGTCAAAAA9590.12646459584737213No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAATTC9110.12013477248900523No Hit
CTTACCTGAGGAGACGGTGACCAGGATTCCCTGGCCCCAGTAGTCAAAAA9050.11934354456920937No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGTCCCAGTAGTCAAAAA8350.11011255217159095No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)