FastQCFastQC Report
Tue 7 May 2024
EGAF00002047691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21629
Total Bases4.5 Mbp
Sequences flagged as poor quality0
Sequence length120-288
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG1078649.86823246567109No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG314114.522169309723058No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTCTGAT14886.879652318646262No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAT12225.649821998243099No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGT10594.896204170326876No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAA8553.9530260298673077No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG8303.8374404734384395No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG4812.2238661056914326No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAT2901.3407924545748764No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGT2901.3407924545748764No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGC900.41610800314392715No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGC650.3005224467150585No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGTCTTGGGCTGACCTAG590.27278191317212996No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGT500.23117111285773728No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGACCTTGGGCTGACCTAG450.20805400157196358No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGATTGGCCTTGGGCTGACCTAG400.18493689028618984No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGC380.17569004577188035No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACCTAG330.15257293448610662No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGTAGACCTGGG240.11096213417171391No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGACCTTGGGCTGACCTAG220.10171528965740441No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGACTGACCTAG220.10171528965740441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)