FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002047692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772750
Sequences flagged as poor quality0
Sequence length43-265
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGAGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCC734999.511355548366225No Hit
GCATGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCG354704.590100291167907No Hit
GCATGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCC349624.524361048204464No Hit
ACATGGGAGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCC238683.08870915561307No Hit
ACATGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCG202202.616628922678745No Hit
ACATGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCC181382.3472015528955033No Hit
GCATGGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCC175622.272662568747978No Hit
GCATGGGGAGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTC157052.0323519896473634No Hit
GCATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTG150781.951213199611776No Hit
GCATGGGAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGT145981.8890973794888386No Hit
GCATGGGAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCA132751.7178906502749918No Hit
GCATGGGATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGC108111.399029440310579No Hit
ACATGGGAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGT101891.3185376900679393No Hit
ACATGGGTCTCCAAGGCGCCTAATTTATCAAGTTTCTATTCGGGACTCTG93161.2055645422193464No Hit
GCATGGGTCACCATTTCCAGAGACAACGCCAAGAATTTACTGTATTTGGA92691.1994823681656421No Hit
GCATGGGTCTCCAAGGCGCCTAATTTATCAAGTTTCTATTCGGGACTCTG87191.1283079909414429No Hit
ACATGGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCC84151.0889679715302492No Hit
GCATGGGTACTGTATTTGGAAATGAGCAGCCTGAGAGCCGAGGACACGGC82051.061792300226464No Hit
ACATGGGAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCA81881.059592364930443No Hit
ACATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTG81731.0576512455516014No Hit
ACATGGGTCACCATTTCCAGAGACAACGCCAAGAATTTACTGTATTTGGA79801.032675509543837No Hit
ACATGGGTACTATGTGGACTCTGTGAGGGGCCGATTCACCATTTCCAGAG79421.0277580071174377No Hit
GCATGGGTCAGCGGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCAT78461.0153348430928502No Hit
ACATGGGTACTGTATTTGGAAATGAGCAGCCTGAGAGCCGAGGACACGGC78361.0140407635069557No Hit
GCATGGGTACTATGTGGACTCTGTGAGGGGCCGATTCACCATTTCCAGAG67170.8692332578453575No Hit
ACATGGGATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGC63370.8200582335813653No Hit
GCATGGGGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAATTTACTG63350.8197994176641863No Hit
GCATGGGGAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTC57330.7418958265933355No Hit
GCATGGGGAGCCGAGGACACGGCCGTCTATTACTGTGCGAGAGATCCCGC50980.6597217728890327No Hit
ACATGGGTCAGCGGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCAT47550.6153348430928502No Hit
GCATGGGTGAGCAGCCTGAGAGCCGAGGACACGGCCGTCTATTACTGTGC43760.5662892267874474No Hit
GCATGGGGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAGGTTGAAG40880.5290197347136849No Hit
GCATGGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCT39400.5098673568424459No Hit
GCATGGGTGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCT39020.5049498544160467No Hit
GCATGGGTCAGAGCCTCGTAAACGGTGATGGAAACACCTACTTGCATTGG37420.48424458104173407No Hit
ACATGGGGAGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTC35540.4599158848269168No Hit
GCATGGGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCC33240.43015205435134257No Hit
GCATGGGGGACAAACCGGCCCCAGACGTTCGGCCAGGGGACCAGGGTGGA33100.42834034293109025No Hit
ACATGGGGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAATTTACTG30990.4010352636687156No Hit
GCATGGGGCAGCCTGAGAGCCGAGGACACGGCCGTCTATTACTGTGCGAG30550.3953413134907797No Hit
GCATGGGACTGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCC29450.3811064380459398No Hit
ACATGGGTGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCT29420.3807182141701715No Hit
GCATGGGGACTGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTC29220.3781300549983824No Hit
GCATGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCATGCAAGGGAC27070.35030734390164997No Hit
ACATGGGTGAGCAGCCTGAGAGCCGAGGACACGGCCGTCTATTACTGTGC26630.344613393723714No Hit
GCATGGGTGCAAGGGACAAACCGGCCCCAGACGTTCGGCCAGGGGACCAG26000.3364606923325784No Hit
GCATGGGTGTCGGGATTTATTACTGCATGCAAGGGACAAACCGGCCCCAG25950.3358136525396312No Hit
ACATGGGGAGCCGAGGACACGGCCGTCTATTACTGTGCGAGAGATCCCGC24670.3192494338401812No Hit
GCATGGGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAATTTACTGT24490.31692009058557097No Hit
GCATGGGGGAAATGAGCAGCCTGAGAGCCGAGGACACGGCCGTCTATTAC24190.31303785182788746No Hit
GCATGGGGATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCG23780.3077321255257198No Hit
ACATGGGTGTCGGGATTTATTACTGCATGCAAGGGACAAACCGGCCCCAG21170.27395664833387257No Hit
GCATGGGAGCATTGAGTTTACTGCAAGGTCAGAAAAGCATGCAAAGCCCT20910.27059204141054677No Hit
ACATGGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCT20700.26787447428016825No Hit
GCATGGGGGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAATTTACT20620.2668392106114526No Hit
ACATGGGGAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTC20340.26321578777094795No Hit
ACATGGGGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAGGTTGAAG20240.2619217081850534No Hit
GCATGGGTCGGGATTTATTACTGCATGCAAGGGACAAACCGGCCCCAGAC19740.2554513102555807No Hit
GCATGGGGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCATGCAAGG19260.24923972824328697No Hit
GCATGGGAGGAATCAGCCCCACTCAGGACACAGCATGGACATGAGGGTCC19190.2483338725331608No Hit
GCATGGGTAGACCAAGATGAAAGTCAGAGATACTATGTGGACTCTGTGAG18200.23552248463280492No Hit
GCATGGGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAGGTTGAAGA18040.23345195729537366No Hit
GCATGGGGGGTCAGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAGG17890.23151083791653188No Hit
ACATGGGACTGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCC17310.22400517631834355No Hit
GCATGGGAAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCC16800.21740537043028144No Hit
GCATGGGGGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCATGCAAG16790.21727596247169198No Hit
GCATGGGTCAGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAGGTTG16650.21546425105143968No Hit
ACATGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCATGCAAGGGAC16580.2145583953413135No Hit
GCATGGGTGGGTCAGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAG16400.21222905208670334No Hit
GCATGGGGTGGACTCTGTGAGGGGCCGATTCACCATTTCCAGAGACAACG16010.20718214170171467No Hit
ACATGGGTCAGAGCCTCGTAAACGGTGATGGAAACACCTACTTGCATTGG15870.20537043028146232No Hit
ACATGGGTACTTGCATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGC15350.19864121643481075No Hit
GCATGGGTCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCA15240.19721772889032674No Hit
ACATGGGTGTATTTGGAAATGAGCAGCCTGAGAGCCGAGGACACGGCCGT15230.1970883209317373No Hit
ACATGGGGACTGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTC15190.1965706890973795No Hit
GCATGGGTACTTGCATTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGC14530.1880297638304756No Hit
ACATGGGGGACAAACCGGCCCCAGACGTTCGGCCAGGGGACCAGGGTGGA14530.1880297638304756No Hit
GCATGGGTGAAAGTCAGAGATACTATGTGGACTCTGTGAGGGGCCGATTC14230.18414752507279197No Hit
GCATGGGTGTGGACTCTGTGAGGGGCCGATTCACCATTTCCAGAGACAAC14060.18194758977677128No Hit
GCATGGGGGGACAAACCGGCCCCAGACGTTCGGCCAGGGGACCAGGGTGG13890.17974765448075056No Hit
GCATGGGTGTATTTGGAAATGAGCAGCCTGAGAGCCGAGGACACGGCCGT13520.1749595600129408No Hit
ACATGGGTGCAAGGGACAAACCGGCCCCAGACGTTCGGCCAGGGGACCAG13480.17444192817858298No Hit
GCATGGGTGAAGATGTCGGGATTTATTACTGCATGCAAGGGACAAACCGG13440.17392429634422518No Hit
ACATGGGGCAGCCTGAGAGCCGAGGACACGGCCGTCTATTACTGTGCGAG13400.17340666450986736No Hit
GCATGGGGAGGACACGGCCGTCTATTACTGTGCGAGAGATCCCGCTTACG13320.1723714008411517No Hit
ACATGGGGGAAATGAGCAGCCTGAGAGCCGAGGACACGGCCGTCTATTAC13150.17017146554513102No Hit
GCATGGGTAAACGGTGATGGAAACACCTACTTGCATTGGTTTCAGCAGAG12990.16810093820769978No Hit
GCATGGGACCATTTCCAGAGACAACGCCAAGAATTTACTGTATTTGGAAA12910.16706567453898413No Hit
ACATGGGAGCATTGAGTTTACTGCAAGGTCAGAAAAGCATGCAAAGCCCT12790.16551277903591072No Hit
GCATGGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCATGCAAGGGA12630.16344225169847945No Hit
ACATGGGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAATTTACTGT12370.16007764477515368No Hit
GCATGGGGTGGGTGGCCAACATAGACCAAGATGAAAGTCAGAGATACTAT12310.15930119702361695No Hit
GCATGGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAG12280.1589129731478486No Hit
ACATGGGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCC12090.156454221934649No Hit
ACATGGGGATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCG12020.1555483662245228No Hit
GCATGGGGAAAATCAGCGGGGTGGAGGTTGAAGATGTCGGGATTTATTAC11710.15153671950824976No Hit
GCATGGGAGCGGCAGTGGGTCAGGCACTGACTTCACACTGAAAATCAGCG11590.14998382400517632No Hit
GCATGGGTGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT11490.1486897444192818No Hit
GCATGGGGGACACGGCCGTCTATTACTGTGCGAGAGATCCCGCTTACGGC11280.14597217728890327No Hit
ACATGGGTGAAAGTCAGAGATACTATGTGGACTCTGTGAGGGGCCGATTC11070.14325461015852475No Hit
GCATGGGGCCTGAGAGCCGAGGACACGGCCGTCTATTACTGTGCGAGAGA11060.1431252021999353No Hit
GCATGGGGGCCGTCTATTACTGTGCGAGAGATCCCGCTTACGGCGCTTTT10960.14183112261404077No Hit
ACATGGGTGTGGACTCTGTGAGGGGCCGATTCACCATTTCCAGAGACAAC10900.14105467486250403No Hit
GCATGGGGGGCTGGAGTGGGTGGCCAACATAGACCAAGATGAAAGTCAGA10870.1406664509867357No Hit
ACATGGGTTCCAGAGACAACGCCAAGAATTTACTGTATTTGGAAATGAGC10710.13859592364930443No Hit
ACATGGGACCATTTCCAGAGACAACGCCAAGAATTTACTGTATTTGGAAA10570.13678421222905207No Hit
GCATGGGAGATCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTC10540.13639598835328373No Hit
GCATGGGGACAACGCCAAGAATTTACTGTATTTGGAAATGAGCAGCCTGA10180.1317373018440634No Hit
ACATGGGAAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCC10080.1304432222581689No Hit
GCATGGGGAGATACTATGTGGACTCTGTGAGGGGCCGATTCACCATTTCC10060.13018440634098996No Hit
ACATGGGTAAACGGTGATGGAAACACCTACTTGCATTGGTTTCAGCAGAG10040.12992559042381108No Hit
GCATGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGA9820.12707861533484308No Hit
GCATGGGGGCTGGAGTGGGTGGCCAACATAGACCAAGATGAAAGTCAGAG9770.12643157554189582No Hit
ACATGGGTGGGTCAGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAG9760.12630216758330637No Hit
GCATGGGTTCCAGAGACAACGCCAAGAATTTACTGTATTTGGAAATGAGC9650.12487868003882238No Hit
GCATGGGAATCAGTCCTACTCAGGACACAGCATGGACATGAGGGTCCCCG9580.12397282432869622No Hit
GCATGGGGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATCAAGTTTCT9570.12384341637010675No Hit
ACATGGGTCGGGATTTATTACTGCATGCAAGGGACAAACCGGCCCCAGAC9530.12332578453574895No Hit
ACATGGGTAGACCAAGATGAAAGTCAGAGATACTATGTGGACTCTGTGAG9480.12267874474280167No Hit
ACATGGGTCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCA9390.1215140731154966No Hit
GCATGGGATGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAG9230.11944354577806535No Hit
GCATGGGGAATCAGTCTCACTCAGGACACAGCATGGACATGAGGGTCCCC9210.11918472986088643No Hit
ACATGGGATGCTTTCTGAGAGTCATGGACCTCCTGTGCAAGAACATGAAG9120.11802005823358136No Hit
ACATGGGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAGGTTGAAGA9120.11802005823358136No Hit
GCATGGGCAGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAGGTTGA8900.11517308314461339No Hit
ACATGGGGGGTCAGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAGG8900.11517308314461339No Hit
ACATGGGGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCATGCAAGG8850.11452604335166613No Hit
ACATGGGGTGGACTCTGTGAGGGGCCGATTCACCATTTCCAGAGACAACG8840.11439663539307668No Hit
ACATGGGGGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAATTTACT8810.11400841151730831No Hit
GCATGGGGTGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCC8710.11271433193141378No Hit
GCATGGGGTGAGGGGCCGATTCACCATTTCCAGAGACAACGCCAAGAATT8690.11245551601423488No Hit
GCATGGGTCAGAGATACTATGTGGACTCTGTGAGGGGCCGATTCACCATT8630.11167906826269815No Hit
GCATGGGGGACTGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCT8610.11142025234551925No Hit
ACATGGGTCAGGCACTGACTTCACACTGAAAATCAGCGGGGTGGAGGTTG8390.10857327725655128No Hit
GCATGGGAGCGGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCATGC8380.10844386929796182No Hit
GCATGGGACTGTATTTGGAAATGAGCAGCCTGAGAGCCGAGGACACGGCC8310.10753801358783564No Hit
GCATGGGCAGCGGGGTGGAGGTTGAAGATGTCGGGATTTATTACTGCATG8260.10689097379488839No Hit
ACATGGGAGATCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTC8190.10598511808476221No Hit
GCATGGGGGATTTATTACTGCATGCAAGGGACAAACCGGCCCCAGACGTT8150.10546748625040439No Hit
GCATGGGGAATTTACTGTATTTGGAAATGAGCAGCCTGAGAGCCGAGGAC7930.10262051116143643No Hit
GCATGGGGAGGTTGAAGATGTCGGGATTTATTACTGCATGCAAGGGACAA7880.10197347136848915No Hit
ACATGGGGTGGGTGGCCAACATAGACCAAGATGAAAGTCAGAGATACTAT7810.10106761565836297No Hit
GCAGGCCAATCTCCAAGGCGCCTAATTTATCAAGTTTCTATTCGGGACTC7760.10042057586541572No Hit
GCATGGGGACAGATTCAGCGGCAGTGGGTCAGGCACTGACTTCACACTGA7750.10029116790682628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)