FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002047693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758315
Sequences flagged as poor quality0
Sequence length51-265
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGTCTGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTG202052.6644600199125694No Hit
GCATGGGTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGC194872.569776412176998No Hit
ACATGGGTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGC172512.2749121407330724No Hit
ACATGGGTCTGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTG172362.2729340709335832No Hit
GCATGGGGAGCACAGGCTACATGGAACTGAGCAGCCTGAGATCTGAAGAC161822.1339416996894434No Hit
GCATGGGTGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTA156292.0610168597482574No Hit
GCATGGGGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCGAG133951.7664163309442644No Hit
GCATGGGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCGA126101.6628973447709725No Hit
ACATGGGTGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTA125901.660259918371653No Hit
GCATGGGGAGATCTGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCA122541.6159511548630847No Hit
GCATGGGAGGGCAGACTCACCTTTACCAGGGACACATCCGCGAGCACAGG119391.5744116890738018No Hit
GCATGGGTCCGCGAGCACAGGCTACATGGAACTGAGCAGCCTGAGATCTG113161.492255856734998No Hit
GCATGGGGAGGCTCCAGGGCAGACTCACCTTTACCAGGGACACATCCGCG107941.4234190277127579No Hit
GCATGGGTACCAGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGC101031.3322959456162675No Hit
ACATGGGTCCGCGAGCACAGGCTACATGGAACTGAGCAGCCTGAGATCTG100421.324251795098343No Hit
GCATGGGCGAGCACAGGCTACATGGAACTGAGCAGCCTGAGATCTGAAGA97321.2833716859088902No Hit
GCATGGGGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGCAGCCT90251.1901386626929442No Hit
ACATGGGGAGCACAGGCTACATGGAACTGAGCAGCCTGAGATCTGAAGAC87121.1488629395435934No Hit
ACATGGGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGGTGTATC86201.1367307781067235No Hit
GCATGGGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGGTGTATC85811.1315877966280503No Hit
ACATGGGGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCGAG81591.075938099602408No Hit
ACATGGGTACCAGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGC81221.0710588607636669No Hit
GCATGGGCAGAGGCTCCAGGGCAGACTCACCTTTACCAGGGACACATCCG81151.070135761523905No Hit
GCATGGGACCCAACAACCACATCCCTCCTCAGAAGCCCCCAGAGCACAAC80141.0568167582073413No Hit
ACATGGGGAGATCTGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCA79631.0500913208890765No Hit
ACATGGGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCGA74980.988771157104897No Hit
GCATGGGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGCAGCCTG68880.9083296519256508No Hit
ACATGGGTACATGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGT62850.8288112459861666No Hit
GCATGGGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGGTGT62130.8193165109486163No Hit
GCATGGGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCGAGG62070.8185252830288205No Hit
GCATGGGTACATGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGT58670.7736890342403882No Hit
GCATGGGGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCG58570.7723703210407284No Hit
GCATGGGGTGGATGGGGTGGCTCGACACTGACAATGGTAAAACAGCATAT58290.7686779240816811No Hit
ACATGGGAGGGCAGACTCACCTTTACCAGGGACACATCCGCGAGCACAGG56020.7387431344494042No Hit
GCATGGGAGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGCAGCC55440.7310945978913775No Hit
GCATGGGGCTCGACACTGACAATGGTAAAACAGCATATTCACAGAGGCTC54710.7214679915338613No Hit
ACATGGGCGAGCACAGGCTACATGGAACTGAGCAGCCTGAGATCTGAAGA54460.7181712085347118No Hit
ACATGGGACCCAACAACCACATCCCTCCTCAGAAGCCCCCAGAGCACAAC52820.6965443120602915No Hit
ACATGGGATCCGCGAGCACAGGCTACATGGAACTGAGCAGCCTGAGATCT50300.6633127394288653No Hit
GCATGGGGGATGGGGTGGCTCGACACTGACAATGGTAAAACAGCATATTC49840.6572466587104303No Hit
ACATGGGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGCAGCCTG49460.6522355485517232No Hit
ACATGGGGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGCAGCCT49420.6517080632718594No Hit
ACATGGGGAGGCTCCAGGGCAGACTCACCTTTACCAGGGACACATCCGCG47520.6266525124783237No Hit
GCATGGGGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGGTG46910.618608361960399No Hit
ACATGGGGTGGATGGGGTGGCTCGACACTGACAATGGTAAAACAGCATAT46850.6178171340406032No Hit
GCATGGGGGTAAAACAGCATATTCACAGAGGCTCCAGGGCAGACTCACCT46850.6178171340406032No Hit
GCATGGGCAGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGCAGC45890.6051574873238694No Hit
ACATGGGGGATGGGGTGGCTCGACACTGACAATGGTAAAACAGCATATTC44760.5902560281677139No Hit
ACATGGGATCTGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGT44430.5859042746088368No Hit
GCATGGGGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCGAGGCG44360.5849811753690749No Hit
ACATGGGACTCACCTTTACCAGGGACACATCCGCGAGCACAGGCTACATG42720.5633542788946546No Hit
GCATGGGACTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAGGGCA41760.5506946321779208No Hit
GCATGGGGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTAC41480.5470022352188735No Hit
GCATGGGATCCGCGAGCACAGGCTACATGGAACTGAGCAGCCTGAGATCT40090.5286721217436026No Hit
GCATGGGATCTGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGT39750.5241884968647594No Hit
ACATGGGGCTCGACACTGACAATGGTAAAACAGCATATTCACAGAGGCTC39080.5153531184270389No Hit
GCATGGGACTCACCTTTACCAGGGACACATCCGCGAGCACAGGCTACATG38330.5054627694295907No Hit
GCATGGGGACAAAGGCCTGAGTGGATGGGGTGGCTCGACACTGACAATGG38030.5015066298306112No Hit
ACATGGGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGGTGT38030.5015066298306112No Hit
GCATGGGCAGCATATTCACAGAGGCTCCAGGGCAGACTCACCTTTACCAG35780.47183558283826643No Hit
ACATGGGGGTAAAACAGCATATTCACAGAGGCTCCAGGGCAGACTCACCT34990.4614177485609542No Hit
GCATGGGGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGGTGTATCCGAA34770.4585165795217027No Hit
ACATGGGGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTAC34400.4536373406829616No Hit
GCATGGGGGCAGACTCACCTTTACCAGGGACACATCCGCGAGCACAGGCT33270.43873588152680615No Hit
ACATGGGGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCGAGGCG32770.43214231552850724No Hit
ACATGGGACTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAGGGCA32490.4284499185694599No Hit
GCATGGGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACT31460.4148671726129643No Hit
ACATGGGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCGAGG31040.4093285771743932No Hit
ACATGGGTACACCTTCACTACCTATTCTATGCATTGGGTGCGCCAGGCCC30580.40326249645595824No Hit
GCATGGGGACACTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAGG30230.39864700025714905No Hit
ACATGGGGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACTGTGCG29550.38967975049946263No Hit
GCATGGGGACTCACCTTTACCAGGGACACATCCGCGAGCACAGGCTACAT29270.38598735354041525No Hit
ACATGGGCAGAGGCTCCAGGGCAGACTCACCTTTACCAGGGACACATCCG29020.3826905705412658No Hit
ACATGGGGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGGTGTATCCGAA28720.37873443094228654No Hit
GCATGGGGGGGTGGCTCGACACTGACAATGGTAAAACAGCATATTCACAG27770.3662066555455187No Hit
ACATGGGGACAAAGGCCTGAGTGGATGGGGTGGCTCGACACTGACAATGG27720.36554729894568877No Hit
ACATGGGTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAGGGCAGA27700.3652835563057569No Hit
GCATGGGTGGGGTGGCTCGACACTGACAATGGTAAAACAGCATATTCACA27370.36093180274687964No Hit
GCATGGGCGACACTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAG27250.35934934690728787No Hit
GCATGGGTACACCTTCACTACCTATTCTATGCATTGGGTGCGCCAGGCCC26740.352623909589023No Hit
GCATGGGTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAGGGCAGA26250.34616221491069016No Hit
ACATGGGGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGGTG26010.34299730323150673No Hit
GCATGGTCTGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGG25760.33970052023235725No Hit
ACATGGGGACACTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAGG25580.3373268364729697No Hit
ACATGGGAGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGCAGCC25140.3315244983944667No Hit
GCATGGGCACTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAGGGC25130.33139262707450073No Hit
ACATGGGGACTCACCTTTACCAGGGACACATCCGCGAGCACAGGCTACAT25010.32981017123490897No Hit
GCATGGGGGCCTGAGTGGATGGGGTGGCTCGACACTGACAATGGTAAAAC24390.3216341493970184No Hit
ACATGGGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATTACT24180.3188648516777329No Hit
GCATGGGAGAGGCTCCAGGGCAGACTCACCTTTACCAGGGACACATCCGC23870.3147768407587876No Hit
GCATGGGTGGATGGGGTGGCTCGACACTGACAATGGTAAAACAGCATATT23650.31187567171953606No Hit
ACATGGGTGGATGGGGTGGCTCGACACTGACAATGGTAAAACAGCATATT23400.30857888872038663No Hit
ACATGGGTGGGGTGGCTCGACACTGACAATGGTAAAACAGCATATTCACA22370.294996142763891No Hit
GCATGGGGGCTCCAGGGCAGACTCACCTTTACCAGGGACACATCCGCGAG21520.2837870805667829No Hit
ACATGGGGGCCTGAGTGGATGGGGTGGCTCGACACTGACAATGGTAAAAC21480.283259595286919No Hit
GCATGGGAACCACATCCCTCCTCAGAAGCCCCCAGAGCACAACGCCTCAC21200.2795671983278717No Hit
GCATGGGACCCTGACGCTGAGCAAAGCAGACTACGAGAAACATTCACAGA20600.271654919129913No Hit
GCATGGGGGCTCGACACTGACAATGGTAAAACAGCATATTCACAGAGGCT19660.25925901505311116No Hit
ACATGGGTACTGTGCGAGGCGTGCAGGTGGTGTATCCGAAATTTTTGACT18880.24897305209576492No Hit
GCATGGGATGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATT18810.2480499528560031No Hit
ACATGGGACCCTGACGCTGAGCAAAGCAGACTACGAGAAACATTCACAGA18650.24594001173654745No Hit
ACATGGGACACCTTCACTACCTATTCTATGCATTGGGTGCGCCAGGCCCC18510.24409381325702378No Hit
ACATGGGCGACACTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAG18470.24356632797715988No Hit
GCATGGGGGGTGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGATGGGGTG17720.2336759789797116No Hit
ACATGGTCTGAAGACACGGCTGTGTATTACTGTGCGAGGCGTGCAGGTGG17360.22892861146093643No Hit
ACATGGGGGGGTGGCTCGACACTGACAATGGTAAAACAGCATATTCACAG16700.2202251043431819No Hit
GCATGGGGAGGCTTGGGTCTCCCCACACACTGCCTGCCAGGACAGTCCTA16580.21864264850359022No Hit
GCATGGGACACCTTCACTACCTATTCTATGCATTGGGTGCGCCAGGCCCC16480.21732393530393043No Hit
ACATGGGGGCTCCAGGGCAGACTCACCTTTACCAGGGACACATCCGCGAG16060.21178533986535938No Hit
GCATGGGGGACAAAGGCCTGAGTGGATGGGGTGGCTCGACACTGACAATG15520.2046642885871966No Hit
GCATGGGCAAGGCTTCTGGATACACCTTCACTACCTATTCTATGCATTGG15330.20215873350784302No Hit
ACATGGGCAGCATATTCACAGAGGCTCCAGGGCAGACTCACCTTTACCAG15300.2017631195479451No Hit
ACATGGGATGGAACTGAGCAGCCTGAGATCTGAAGACACGGCTGTGTATT15160.19991692106842143No Hit
GCATGGGGAGATCTGAAGACACGGCTGTGTATTACTGTGTGAGGCGTGCA14990.19767510862899984No Hit
ACATGGGGGCAGACTCACCTTTACCAGGGACACATCCGCGAGCACAGGCT14910.19662013806927198No Hit
GCATGGGGACCCAACAACCACATCCCTCCTCAGAAGCCCCCAGAGCACAA14900.19648826674930603No Hit
ACATGGGCAGGGACACATCCGCGAGCACAGGCTACATGGAACTGAGCAGC14450.19055405735083705No Hit
ACATGGGCACTGACAATGGTAAAACAGCATATTCACAGAGGCTCCAGGGC14400.18989470075100717No Hit
GCATGGGGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCT13790.18185055023308255No Hit
ACATGGGGGCTCGACACTGACAATGGTAAAACAGCATATTCACAGAGGCT13650.18000435175355886No Hit
ACATGGGTATTCACAGAGGCTCCAGGGCAGACTCACCTTTACCAGGGACA13500.17802628195406922No Hit
ACATGGGAGAGGCTCCAGGGCAGACTCACCTTTACCAGGGACACATCCGC13060.17222394387556622No Hit
ACATGGGGGGTGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGATGGGGTG13040.17196020123563427No Hit
ACATGGGTGCATTGGGTGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGAT13030.1718283299156683No Hit
GCATGGGTACTGTGCGAGGCGTGCAGGTGGTGTATCCGAAATTTTTGACT11780.15534441491992113No Hit
GCATGGGTGCATTGGGTGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGAT11550.15231137456070365No Hit
ACATGGGAACCACATCCCTCCTCAGAAGCCCCCAGAGCACAACGCCTCAC11500.15165201796087377No Hit
ACATGGGGGACAAAGGCCTGAGTGGATGGGGTGGCTCGACACTGACAATG11370.14993769080131608No Hit
ACATGGGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTT10740.1416297976434595No Hit
GCATGGGAAGGCTTCTGGATACACCTTCACTACCTATTCTATGCATTGGG10110.13332190448560297No Hit
GCATGGGGGTGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGATGGGGTGG9940.13108009204618132No Hit
ACATGGGGAGATCTGAAGACACGGCTGTGTATTACTGTGTGAGGCGTGCA9390.12382716944805258No Hit
GCATGGGTATTCACAGAGGCTCCAGGGCAGACTCACCTTTACCAGGGACA9160.1207941290888351No Hit
ACATGGGGACCCAACAACCACATCCCTCCTCAGAAGCCCCCAGAGCACAA8740.11525553365026406No Hit
GCATGGGCATATTCACAGAGGCTCCAGGGCAGACTCACCTTTACCAGGGA8390.11064003745145487No Hit
GCATGGGGATGGGGTGGCTCGACACTGACAATGGTAAAACAGCATATTCA8320.10971693821169304No Hit
ACATGGGACTACCTATTCTATGCATTGGGTGCGCCAGGCCCCCGGACAAA8280.10918945293182912No Hit
ACATGGGGGTGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGATGGGGTGG7880.10391460013319004No Hit
GCATGGGAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGAGG7880.10391460013319004No Hit
GCATGGGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTT7740.10206840165366635No Hit
GCATGGGGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGATGGGGTGGCTC7730.10193653033370037No Hit
ACATGGGGGGGCCTCAGTGAAGGTTTCCTGCAAGGCTTCTGGATACACCT7710.10167278769376842No Hit
GCATGGGTGCGCCAGGCCCCCGGACAAAGGCCTGAGTGGATGGGGTGGCT7630.1006178171340406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)