FastQCFastQC Report
Wed 8 May 2024
EGAF00002047695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217016
Total Bases43.8 Mbp
Sequences flagged as poor quality0
Sequence length73-288
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATGGTCAGAGC12403157.15292881630848No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA5267524.272403878055073No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA158797.316972020496185No Hit
CTTACCTGAGGAGACGGTGACCGACTTCCCTGGCCCAGGATGAGTGCGAG42011.9358019685184504No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA30191.3911416669738637No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAAATATCAAAAG23971.104526855162753No Hit
CTTACCTGAGGAGACGGTGACCGACGTGCTGTAGACGCGCAGGCCGCTCA22871.0538393482508202No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG14790.681516570206805No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATGGTCAGAGT5830.2686437866332436No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG5440.2506727614553766No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAG5440.2506727614553766No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGACCCCAATGGTCAGAGC5220.24053526007299003No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG4600.2119659379953552No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAG4510.20781877833892431No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCCGGCCCCAATGGTCAGAGC3280.15114092970103585No Hit
CTTACCTGAGGAGACGGTGACCGGGGTTCCCTGGCCCCAATGGTCAGAGC2340.10782615106720242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)