FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002047699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172965
Sequences flagged as poor quality0
Sequence length45-265
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGGGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAG112136.482814442228197No Hit
GCATGGGTGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACC97345.627728153094556No Hit
GCATGGGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAGAG68513.9609169485155955No Hit
GCATGGGGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAGA64323.71867140751019No Hit
GCATGGGACTGCAACCCTGTGCCCGTCATGCCCAGCAGGCTCCTGCTCCA58443.3787182377937737No Hit
ACATGGGTGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACC52863.056109617552684No Hit
GCATGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTTT51993.0058104240742347No Hit
ACATGGGGGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAG51382.9705431734744026No Hit
GCATGGGGGTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGAC40212.3247477813430466No Hit
GCATGGGAGCCCAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGG35202.0350938051050793No Hit
ACATGGGGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAGA35102.029312288613303No Hit
ACATGGGGGTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGAC31051.7951608706963835No Hit
ACATGGGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAGAG29401.699765848582083No Hit
ACATGGGACTGCAACCCTGTGCCCGTCATGCCCAGCAGGCTCCTGCTCCA27331.5800884572023242No Hit
ACATGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTTT26541.5344144769172954No Hit
GCATGGGACCTTCTCAAGCACTTTGAGTGACCACGGCTTGCAAGCTGGTG26161.5124447142485473No Hit
GCATGGGTATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGA25881.4962564680715753No Hit
GCATGGGGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCG21661.2522764721186368No Hit
ACATGGGACCTTCTCAAGCACTTTGAGTGACCACGGCTTGCAAGCTGGTG21331.2331974676957767No Hit
GCATGGGGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAGAGAGG20261.1713352412337756No Hit
ACATGGGTATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGA19941.1528343884600931No Hit
ACATGGGAGCCCAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGG15570.900182117769491No Hit
GCATGGGCTGACAACACGGCCTCCCTGACAATCTCTGGACTCCAACCTGA15120.8741652935565No Hit
GCATGGGGAGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTA13540.7828173329864423No Hit
GCATGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGC11870.6862660075737866No Hit
GCATGGGTCGCTTCTCTGGGTCCAGGTCTGACAACACGGCCTCCCTGACA11540.6671870031509265No Hit
GCATGGGACACACTGGGGTAACTCCAAGGCTGTGTGTGGCACTTGCCTTG10210.5902928338103085No Hit
ACATGGGTCTCTGGACTCCAACCTGAGGACGAGGCTGATTATTATTGCTG9840.568901222790738No Hit
GCATGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGG9130.5278524556991299No Hit
GCATGGGGAGGCTTGGGTCTCCCCACACACTGCCTGCCAGGACAGTCCTA9010.5209146359089989No Hit
GCATGGGGGACTCCAACCTGAGGACGAGGCTGATTATTATTGCTGCTCAT8250.4769751105715029No Hit
GCATGGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAGAGA8100.46830283583383925No Hit
GCATGGGTGAGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGT7980.4613650160437082No Hit
ACATGGGGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCG7930.45847425779782036No Hit
GCATGGGATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC7800.4509582863585118No Hit
GCATGGGAGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAG7800.4509582863585118No Hit
GCATGGGTCTCTGGACTCCAACCTGAGGACGAGGCTGATTATTATTGCTG7630.44112970832249304No Hit
ACATGGGACACACTGGGGTAACTCCAAGGCTGTGTGTGGCACTTGCCTTG7430.42956667533894144No Hit
GCATGGGTAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGGTCCAGG7010.4052843060734831No Hit
ACATGGGTCGCTTCTCTGGGTCCAGGTCTGACAACACGGCCTCCCTGACA6810.3937212730899315No Hit
GCATGGGGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAA6800.3931431214407539No Hit
GCATGGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT6300.36423553898187494No Hit
ACATGGGGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAGAGAGG6100.3526725059983234No Hit
GCATGGGTCCAACCTGAGGACGAGGCTGATTATTATTGCTGCTCATATGC5950.34400023126065965No Hit
ACATGGGATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCC5820.33648425982135116No Hit
GCATGGGTGGGGAGATATAACCTTGTCTCCTGGTACCAACAACATCCAGG5800.33532795652299596No Hit
GCATGGGGGCCTCCCTGACAATCTCTGGACTCCAACCTGAGGACGAGGCT5770.33359350157546325No Hit
GCATGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTAGAGAGGT5600.3237649235394444No Hit
ACATGGGGGACTCCAACCTGAGGACGAGGCTGATTATTATTGCTGCTCAT5440.31451449715260316No Hit
GCATGGGACCTGAGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGT5440.31451449715260316No Hit
GCATGGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTCCTACGT5210.3012170092215188No Hit
ACATGGGCTGACAACACGGCCTCCCTGACAATCTCTGGACTCCAACCTGA5110.295435492729743No Hit
GCATGGGCGGCCTCCCTGACAATCTCTGGACTCCAACCTGAGGACGAGGC4700.2717312751134623No Hit
GCATGGGGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAC4680.2705749718151071No Hit
GCATGGGACGGCCTCCCTGACAATCTCTGGACTCCAACCTGAGGACGAGG4600.26594975862168646No Hit
GCATGGAGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAGAG4510.26074639377908826No Hit
GCATGGGTGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGG4360.25207411904142457No Hit
ACATGGGTCCAACCTGAGGACGAGGCTGATTATTATTGCTGCTCATATGC4360.25207411904142457No Hit
ACATGGGGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAA4360.25207411904142457No Hit
ACATGGGAATCTCTGGACTCCAACCTGAGGACGAGGCTGATTATTATTGC4300.24860520914635909No Hit
ACATGGGATTGCTGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGG4300.24860520914635909No Hit
GCATGGGGGGTCCAGGTCTGACAACACGGCCTCCCTGACAATCTCTGGAC4210.24340184430376088No Hit
GCATGGGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCGT4160.24051108605787297No Hit
GCATGGGGAGGTCAATAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTG4140.23935478275951783No Hit
GCATGGGAATCTCTGGACTCCAACCTGAGGACGAGGCTGATTATTATTGC4130.23877663111034025No Hit
ACATGGGTGAGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGT4120.23819847946116268No Hit
GCATGGGTAACCTTGTCTCCTGGTACCAACAACATCCAGGCAAAGCCCCC4070.23530772121527474No Hit
GCATGGGGACAACACGGCCTCCCTGACAATCTCTGGACTCCAACCTGAGG3980.23010435637267654No Hit
ACATGGGTAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGGTCCAGG3930.22721359812678865No Hit
GCATGGGGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGGTCCAGGTCTGA3910.22605729482843348No Hit
GCATGGGATTGCTGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGG3910.22605729482843348No Hit
ACATGGGGAGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTA3790.21911947503830254No Hit
GCATGGGCAACACGGCCTCCCTGACAATCTCTGGACTCCAACCTGAGGAC3690.21333795854652673No Hit
GCATGGGTGACAACACGGCCTCCCTGACAATCTCTGGACTCCAACCTGAG3620.20929089700228368No Hit
ACATGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGC3590.207556442054751No Hit
GCATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG3500.20235307721215276No Hit
ACATGGGACCTGAGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGT3490.2017749255629752No Hit
ACATGGGTAACCTTGTCTCCTGGTACCAACAACATCCAGGCAAAGCCCCC3390.1959934090711994No Hit
ACATGGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAGAGA3360.19425895412366664No Hit
GCATGGGTCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCT3360.19425895412366664No Hit
ACATGGGGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGGTCCAGGTCTGA3340.19310265082531147No Hit
GCATGGGTCTGGACTCCAACCTGAGGACGAGGCTGATTATTATTGCTGCT3340.19310265082531147No Hit
ACATGGGGGCCTCCCTGACAATCTCTGGACTCCAACCTGAGGACGAGGCT3330.1925244991761339No Hit
GCATGGGTGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCA3200.18500852773682536No Hit
ACATGGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGT3190.18443037608764778No Hit
GCATGGGAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTC3190.18443037608764778No Hit
ACATGGGGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAC3010.17402364640245138No Hit
ACATGGGTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGC3010.17402364640245138No Hit
GCATGGGTGCTGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGA3000.17344549475327378No Hit
GCATGGGGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGGTCCAGGTCT2950.1705547365073859No Hit
GCATGGGTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGC2920.16882028155985315No Hit
ACATGGGTCACTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCT2860.16535137166478767No Hit
GCATGGGCAAACTCTTGATTTCTGAGGTCAATAAGCGGCCCTCAGGGGTT2790.1613043101205446No Hit
ACATGGGTGGGGAGATATAACCTTGTCTCCTGGTACCAACAACATCCAGG2780.16072615847136704No Hit
GCATGGGCTGGGTCCAGGTCTGACAACACGGCCTCCCTGACAATCTCTGG2760.1595698551730119No Hit
ACATGGGAGGACGAGGCTGATTATTATTGCTGCTCATATGCAGGTGGTAG2710.15667909692712398No Hit
GCATGGGTGCAGGTGGCAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACC2700.1561009452779464No Hit
ACATGGGTGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGACCA2700.1561009452779464No Hit
ACATGGGGAGGCTTGGGTCTCCCCACACACTGCCTGCCAGGACAGTCCTA2680.15494464197959126No Hit
ACATGGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGG2670.15436649033041366No Hit
GCATGGGGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCA2630.15205388373370335No Hit
GCATGGGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGGTCCAGGTCTG2610.15089758043534818No Hit
GCATGGGGGGAGATATAACCTTGTCTCCTGGTACCAACAACATCCAGGCA2610.15089758043534818No Hit
ACATGGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTCCTACGT2600.1503194287861706No Hit
GCATGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTTTCTGATC2580.14916312548781546No Hit
GCATGGGGATTGCTGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAG2420.13991269910097417No Hit
GCATGGGACCAGTGATGTGGGGAGATATAACCTTGTCTCCTGGTACCAAC2390.13817824415344146No Hit
ACATGGGTGACAACACGGCCTCCCTGACAATCTCTGGACTCCAACCTGAG2290.13239672766166566No Hit
ACATGGGTCTGGACTCCAACCTGAGGACGAGGCTGATTATTATTGCTGCT2250.13008412106495534No Hit
GCATGGGGGTCCAGGTCTGACAACACGGCCTCCCTGACAATCTCTGGACT2210.12777151446824503No Hit
GCATGGGTCTGGGTCCAGGTCTGACAACACGGCCTCCCTGACAATCTCTG2170.12545890787153471No Hit
ACATGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTAGAGAGGT2130.12314630127482437No Hit
GCATGGGGATCGCTTCTCTGGGTCCAGGTCTGACAACACGGCCTCCCTGA2110.12198999797646923No Hit
GCATGGGGTCCAGGTCTGACAACACGGCCTCCCTGACAATCTCTGGACTC2040.11794293643222617No Hit
GCATGGGGGTGGTAGAGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC2030.1173647847830486No Hit
GCATGGGTCTGAGGTCAATAAGCGGCCCTCAGGGGTTTCTGATCGCTTCT1920.11100511664209523No Hit
ACATGGGACGGCCTCCCTGACAATCTCTGGACTCCAACCTGAGGACGAGG1910.11042696499291764No Hit
ACATGGGTGCTGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAGGGA1880.10869251004538491No Hit
ACATGGGTGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGG1860.10753620674702974No Hit
GCATGGGGCTTCTCTGGGTCCAGGTCTGACAACACGGCCTCCCTGACAAT1860.10753620674702974No Hit
GCATGGGGCCCCCAAACTCTTGATTTCTGAGGTCAATAAGCGGCCCTCAG1820.10522360015031942No Hit
ACATGGGGACAACACGGCCTCCCTGACAATCTCTGGACTCCAACCTGAGG1820.10522360015031942No Hit
ACATGGGCGGCCTCCCTGACAATCTCTGGACTCCAACCTGAGGACGAGGC1780.10291099355360911No Hit
ACATGGGGATTGCTGCTCATATGCAGGTGGTAGAGAGGTGTTCGGCGGAG1770.10233284190443154No Hit
ACATGGGGATCGCTTCTCTGGGTCCAGGTCTGACAACACGGCCTCCCTGA1760.10175469025525395No Hit
ACATGGGTCTGGGTCCAGGTCTGACAACACGGCCTCCCTGACAATCTCTG1740.1005983869568988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)