FastQCFastQC Report
Wed 8 May 2024
EGAF00002047703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128048
Total Bases23.1 Mbp
Sequences flagged as poor quality0
Sequence length45-265
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAAA33402.608396851180807No Hit
GCATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCC28092.1937086092715234No Hit
GCATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTC27032.1109271523178808No Hit
GCATGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCT26972.106241409471448No Hit
GCATGGGATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACC26572.0750031238285644No Hit
GCATGGGGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTC25792.0140884668249406No Hit
GCATGGGGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAA24651.9250593527427216No Hit
GCATGGGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGA24271.8953829813819818No Hit
GCATGGGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGAC22251.737629638885418No Hit
GCATGGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCC19101.4916281394477071No Hit
ACATGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCT19061.4885043108834186No Hit
GCATGGGGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCAT18691.459608896663751No Hit
ACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCC17781.3885417968261902No Hit
GCATGGGTAGTGGGACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCT17441.361989254029739No Hit
GCATGGGCAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT17361.3557415969011621No Hit
ACATGGGATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACC17181.3416843683618644No Hit
ACATGGGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAAA16381.2792077970760964No Hit
GCATGGGTCAGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGC16151.2612457828314383No Hit
GCATGGGGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAG15521.212045482943896No Hit
GCATGGGTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGA14661.1448831688116956No Hit
ACATGGGGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGA13431.0488254404598276No Hit
ACATGGGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGAC13271.036330126202674No Hit
ACATGGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCC12650.9879107834562039No Hit
GCATGGGGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGA12430.9707297263526179No Hit
GCATGGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTC12100.9449581406972385No Hit
GCATGGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCT11740.916843683618643No Hit
ACATGGGGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCAT11300.8824815694114706No Hit
GCATGGGACTGTGCATCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGG10960.8559290266150192No Hit
ACATGGGGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAA10690.8348431838060727No Hit
ACATGGGTCAGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGC10140.7918905410471074No Hit
GCATGGGCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATG10130.7911095839060353No Hit
GCATGGGGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGC9970.7786142696488817No Hit
GCATGGGGGCCATCAGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTAC9740.7606522554042234No Hit
GCATGGGCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGACAG9440.7372235411720605No Hit
GCATGGGACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAG9330.7286330126202674No Hit
ACATGGGTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGA9320.7278520554791953No Hit
GCATGGGGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATC9180.7169186555041859No Hit
GCATGGGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATG9050.7067662126702486No Hit
GCATGGGGACCGAATCTCTGTCTCCAAGTCTGGCACCTCAGCCTCCCTGG9040.7059852555291766No Hit
ACATGGGTAGTGGGACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCT8520.6653754841934274No Hit
GCATGGGGTGCATCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGG8470.6614706984880669No Hit
ACATGGGACTGTGCATCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGG8450.6599087842059228No Hit
ACATGGGGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTC8190.6396038985380482No Hit
ACATGGGGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGA8050.6286704985630389No Hit
GCATGGGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGAGTGG7640.5966512557790828No Hit
ACATGGGGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAG7630.5958702986380108No Hit
GCATGGGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCC7350.574003498687992No Hit
GCATGGGAGGGGGACGGCGGATTCGGCGGAGGGACCAAACTGACCGTCCT7280.5685367987004873No Hit
ACATGGGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATG7070.5521366987379732No Hit
ACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTC7030.5490128701736848No Hit
GCATGGGGGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCT6880.5372985130576033No Hit
GCATGGGGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGAGTG6850.5349556416343871No Hit
GCATGGGCAGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCA6590.5146507559665126No Hit
ACATGGGGGCCATCAGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTAC6180.48263151318255654No Hit
GCATGGGAGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCAT6100.4763838560539797No Hit
ACATGGGGTGCATCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGG5940.4638885417968262No Hit
GCATGGGGGGGTCCCGGACCGAATCTCTGTCTCCAAGTCTGGCACCTCAG5860.45764088466824937No Hit
GCATGGGGCATCAAGGTTGATACCTACAGCATATAGTGGGACCAGCAGAA5700.44514557041109587No Hit
ACATGGGATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCCGGTGTT5510.430307384730726No Hit
ACATGGGGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATC5450.42562164188429336No Hit
GCATGGGGAGGACAGTCCTACAGGATGAGCAGGGGACCCACAGTTCACGG5440.4248406847432213No Hit
ACATGGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTC5260.4107834562039235No Hit
GCATGGGGGACCGAATCTCTGTCTCCAAGTCTGGCACCTCAGCCTCCCTG5260.4107834562039235No Hit
GCATGGGGCATCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAG5190.4053167562164188No Hit
GCATGGGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCA5170.40375484193427463No Hit
ACATGGGACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAG5100.39828814194677No Hit
GCATGGGGAGCATCAGCCAGTCTCACCTGCAGTATACGCAGTGGCATCAA5030.39282144195926527No Hit
ACATGGGGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGC4790.37407847057353494No Hit
ACATGGGGACCGAATCTCTGTCTCCAAGTCTGGCACCTCAGCCTCCCTGG4730.36939272772710235No Hit
GCATGGGGTGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGG4620.36080219917530926No Hit
GCATGGGGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAAACTGA4620.36080219917530926No Hit
GCATGGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT4450.3475259277770836No Hit
GCATGGGAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAGATGAGGCT4340.33893539922529053No Hit
GCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAAACT4300.33581157066100215No Hit
GCATGGGTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAGATGAGG4120.3217543421217044No Hit
ACATGGGCAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT4000.3123828564288392No Hit
ACATGGGCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATG3990.3116018992877671No Hit
GCATGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAACATCG3920.3061351993002624No Hit
GCATGGGATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCCGGTGTT3820.2983256278895414No Hit
ACATGGGAGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCAT3800.29676371360739723No Hit
GCATGGGGAGTGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTA3770.2944208421841809No Hit
GCATGGGGAACGGCCCCCAAACTCCTCATGTATAATAATAATCAGCGGCC3740.29207797076096464No Hit
ACATGGGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGAGTGG3710.2897350993377483No Hit
GCATGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTTT3700.28895414219667626No Hit
GCATGGGATGTATAATAATAATCAGCGGCCCTCAGGGGTCCCGGACCGAA3610.28192552792702735No Hit
ACATGGGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCATCA3610.28192552792702735No Hit
GCATGGGGGCCCTCAGGGGTCCCGGACCGAATCTCTGTCTCCAAGTCTGG3530.2756778707984506No Hit
ACATGGGGGCCCTCAGGGGTCCCGGACCGAATCTCTGTCTCCAAGTCTGG3520.2748969136573785No Hit
GCATGGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAGAT3460.27021117081094587No Hit
GCATGGGGGCCATCAGTGGGCTCCGGTCGGAAGGTGAGGCTGATTATTAC3420.2670873422466575No Hit
GCATGGGGATTATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCCGG3340.2608396851180807No Hit
ACATGGGGCATCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAG3220.25146819942521553No Hit
ACATGGGGATTATTACTGTGCATCATGGGATGACAGCCTGAGTGGTCCGG3180.24834437086092717No Hit
ACATGGGGGACCGAATCTCTGTCTCCAAGTCTGGCACCTCAGCCTCCCTG3160.24678245657878298No Hit
ACATGGGGCATCAAGGTTGATACCTACAGCATATAGTGGGACCAGCAGAA3140.24522054229663875No Hit
GCATGGGGGGGACCCAGACCCTGGAGCCTCAGTTGGAAGCAGCCGATCCA3110.24287767087342246No Hit
GCATGGGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAGA3090.24131575659127827No Hit
ACATGGGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGACAGCC3090.24131575659127827No Hit
ACATGGGCGGAAGATGAGGCTGATTATTACTGTGCATCATGGGATGACAG3090.24131575659127827No Hit
ACATGGGAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAGATGAGGCT3080.24053479945020617No Hit
GCATGGGGGAGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCC2980.2327252280394852No Hit
ACATGGGGTTTTGGTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACT2900.22647757091090842No Hit
ACATGGGAGGGGGACGGCGGATTCGGCGGAGGGACCAAACTGACCGTCCT2850.22257278520554793No Hit
ACATGGGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCA2800.21866799950018742No Hit
ACATGGGGTGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGG2790.21788704235911532No Hit
ACATGGGGGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCT2770.21632512807697116No Hit
ACATGGGTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAGATGAGG2680.20929651380732225No Hit
ACATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAAACT2660.20773459952517806No Hit
ACATGGGATGTATAATAATAATCAGCGGCCCTCAGGGGTCCCGGACCGAA2630.20539172810196174No Hit
GCATGGGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCA2590.20226789953767338No Hit
ACATGGGGGGGTCCCGGACCGAATCTCTGTCTCCAAGTCTGGCACCTCAG2550.199144070973385No Hit
ACATGGGCAGTGGGCTCCGGTCGGAAGATGAGGCTGATTATTACTGTGCA2520.19680119955016867No Hit
ACATGGGGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAAACTGA2430.1897725852805198No Hit
GCATGGGTCTGTCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTG2400.1874297138573035No Hit
ACATGGGGAGGCTGATTATTACTGTGCATCATGGGATGACAGCCTGAGTG2390.1866487567162314No Hit
GCATGGGAATCTCTGTCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATC2360.18430588529301511No Hit
ACATGGGTCTGTCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTG2300.17962014244658253No Hit
GCATGGGGAGTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG2200.17181057103586156No Hit
ACATGGGAATCTCTGTCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATC2180.17024865675371736No Hit
ACATGGGGGCCATCAGTGGGCTCCGGTCGGAAGGTGAGGCTGATTATTAC2160.16868674247157317No Hit
GCATGGGGGCCCCCAAACTCCTCATGTATAATAATAATCAGCGGCCCTCA2160.16868674247157317No Hit
ACATGGGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCG2090.16322004248406846No Hit
ACATGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTTT2060.1608771710608522No Hit
ACATGGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCT2060.1608771710608522No Hit
GCATGGGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCG1990.1554104710733475No Hit
ACATGGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAGAT1930.1507247282269149No Hit
GCATGGGAGAAAGGAGACAGTCTCTTAGGGCAGAGATGTGTCTGTCCCTG1920.1499437710858428No Hit
ACATGGGTCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCT1880.14681994252155442No Hit
ACATGGGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCGGAAGA1810.14135324253404974No Hit
GCATGGGGTTTTGGTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACT1790.13979132825190554No Hit
GCATGGGTCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCT1730.13510558540547293No Hit
ACATGGGGAGCATCAGCCAGTCTCACCTGCAGTATACGCAGTGGCATCAA1690.13198175684118457No Hit
ACATGGGGAGTGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTA1630.127296013994752No Hit
GCATGGGGGTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGAC1610.12573409971260777No Hit
GCATGGGGGACGTAATTATGTATACTGGTACCAGCAATTCCCAGGAACGG1610.12573409971260777No Hit
ACATGGGGAACGGCCCCCAAACTCCTCATGTATAATAATAATCAGCGGCC1580.12339122828939149No Hit
GCATGGGAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGT1570.12261027114831938No Hit
GCATGGGGGTCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTC1480.1155816568786705No Hit
ACATGGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT1400.10933399975009371No Hit
GCATGGGGTCCCGGACCGAATCTCTGTCTCCAAGTCTGGCACCTCAGCCT1340.10464825690366113No Hit
GCATGGGATGTATACTGGTACCAGCAATTCCCAGGAACGGCCCCCAAACT1320.10308634262151695No Hit
ACATGGGGTCCCGGACCGAATCTCTGTCTCCAAGTCTGGCACCTCAGCCT1310.10230538548044482No Hit
GCATGGGAACGGCCCCCAAACTCCTCATGTATAATAATAATCAGCGGCCC1300.10152442833937274No Hit
GCATGGGGGGTCCCGGACCGAATCTCTGTCTCCAAGTCTGGCACCTCAGC1300.10152442833937274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)