FastQCFastQC Report
Wed 8 May 2024
EGAF00002047704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254094
Total Bases49.9 Mbp
Sequences flagged as poor quality0
Sequence length101-288
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAT6974527.44850330979874No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG4320017.001582091666865No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG2900011.413099089313404No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGC219648.644045117161365No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGG211638.328807449211709No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGC152556.003683676119861No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGG143015.628232071595551No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG83813.298385636811574No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG63262.4896298220343653No Hit
CTTACCTGAGGAGACGGTGACCAGGGTACCCTGGCCCCAGTAGTCGAAAG23830.9378419010287532No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA21120.8311884578148245No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGT16830.6623533023211882No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGT13390.5269703338134706No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA11490.45219485702141726No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA11270.4435366439191795No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAAT8780.3455414138074886No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC8060.3172054436547105No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAACGT7940.3124827819625808No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGGAGTCAAGGA7580.2983147968861917No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTCAGTCATGG6950.2735208230025109No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAG4910.19323557423630625No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAG4610.18142892000598204No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT4080.16057049753240926No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGTAGCCACAGTTCGTTTGAT3470.13656363393075005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)