FastQCFastQC Report
Wed 8 May 2024
EGAF00002047705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537640
Total Bases107.8 Mbp
Sequences flagged as poor quality0
Sequence length68-288
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGTAGACCTGGG23022842.82196265158842No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGT13307224.751134588200284No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG6552012.186593259430103No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGT409337.613458820028271No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG241094.484227364035414No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGC46520.8652630012647868No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGC24350.45290528978498623No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTCC21690.403429804330035No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG15920.29610892046722714No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACCTGT14540.2704411874116509No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGTAGCCTTGGGCTGACCTGT13660.25407335763708055No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACCTAG11820.21984971356297894No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAG11650.21668774644743694No Hit
CTTACCTGAGGAGACGGTGACCGGGGTCCCCTGGCCCCAGTAGACCTGGG11060.20571386057585003No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAG9210.17130421843612825No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG7840.14582248344617216No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTCC6950.1292686556059817No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCTGGCCCCAGTAGACCTGGGA6780.1261066884904397No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGTAGCCTTGGGCTGACCTAG6110.11364481809389183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)