FastQCFastQC Report
Tue 7 May 2024
EGAF00002047709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21629
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length97-265
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGGACACACACTGGGGTAACTCCAAGGCTGTGTGTGGCACTTGCC456821.11979287068288No Hit
GCATGGGCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCT398418.41971427250451No Hit
ACATGGGGACACACACTGGGGTAACTCCAAGGCTGTGTGTGGCACTTGCC223710.342595589255167No Hit
ACATGGGCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCT19959.223727403023718No Hit
GCATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG4321.9973184150908503No Hit
GCATGGGAGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCC2941.3592861436034953No Hit
GCATGGGAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGT2671.2344537426603173No Hit
ACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG2491.151232142031532No Hit
GCATGGGTATGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCA2281.054140274631282No Hit
ACATGGGAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGT1840.8507096953164733No Hit
GCATGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCC1570.7258772943732952No Hit
GCATGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCG1550.7166304498589856No Hit
GCATGGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCAT1350.6241620047158907No Hit
GCATGGGGACACACACCGGGGTAACTCCAAGGCTGTGTGTGGCACTTGCC1010.46696564797262935No Hit
ACATGGGTATGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCA980.45309538120116516No Hit
GCATGGGTCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTG910.4207314254010819No Hit
GCATGGGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTG860.3976143141153081No Hit
ACATGGGAGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCC840.38836746960099866No Hit
GCATGGGACACAGCTGCAGTGGGGGCTGGGGCTGGGGCATCCCAGGGAGG820.37912062508668914No Hit
GCATGGGGGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAG810.37449720282953447No Hit
GCATGGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCC710.328262980257987No Hit
GCATGGGAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCA700.3236395580008322No Hit
ACATGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCC670.30976929122936797No Hit
GCATGGGGAGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTC660.30514586897221324No Hit
ACATGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCG660.30514586897221324No Hit
GCATGGGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCC570.2635350686578205No Hit
GCATGGGCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCC570.2635350686578205No Hit
GCATGGGGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCT560.2589116464006658No Hit
GCATGGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT540.24966480188635629No Hit
GCATGGGTGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAAG540.24966480188635629No Hit
ACATGGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCAT520.2404179573720468No Hit
GCATGGGTCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCT460.21267742382911833No Hit
GCATGGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCG450.20805400157196358No Hit
GCATGGGATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGC440.20343057931480882No Hit
ACATGGGGACACACACCGGGGTAACTCCAAGGCTGTGTGTGGCACTTGCC430.19880715705765406No Hit
GCATGGGAGTCCAGGGAGAGGACAGAGCCAATGGACTGGGGTGTACTGTA420.19418373480049933No Hit
GCATGGGAGACTGAGGATGAGGCTGATTATTACTGCTTCTCATATGCAGG400.18493689028618984No Hit
GCATGGGATCGAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTC400.18493689028618984No Hit
ACATGGGACACAGCTGCAGTGGGGGCTGGGGCTGGGGCATCCCAGGGAGG400.18493689028618984No Hit
GCATGGGAGGACATAGTGGAAAACACCCTGACCCCTCTGCCTGGCATAGA350.1618197790004161No Hit
ACATGGGGGAATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCC350.1618197790004161No Hit
ACATGGGAGTCCAGGGAGAGGACAGAGCCAATGGACTGGGGTGTACTGTA340.15719635674326138No Hit
GCATGGGGCACTTGCCTTGGACAGCAGACAGGCACAGGGACACCTCTAGG340.15719635674326138No Hit
GCATGGGGCAGGCAGCTACACCCATTGGGTGTTCGGCGGAGGGACCAAGC340.15719635674326138No Hit
CCATGGGCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCT340.15719635674326138No Hit
GCATGGGAACAGTCTGCAATCTGAAGATTTTGCAACTTACTACTGTCAAC330.15257293448610662No Hit
GCATGGGACTGCTTCTCATATGCAGGCAGCTACACCCATTGGGTGTTCGG330.15257293448610662No Hit
ACATGGGTCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTG320.14794951222895186No Hit
GCATGGGAGTCTGCAATCTGAAGATTTTGCAACTTACTACTGTCAACAGA290.13407924545748764No Hit
GCATGGGGACACACACTGGGGTAACTCCATGGCTGTGTGTGGCACTTGCC270.12483240094317814No Hit
ACATGGGGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTG260.1202089786860234No Hit
GCATGGGGGTCACAGTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGT250.11558555642886864No Hit
GCATGGGGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAAG250.11558555642886864No Hit
GCATGGGGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCCC250.11558555642886864No Hit
CCATGGGGACACACACTGGGGTAACTCCAAGGCTGTGTGTGGCACTTGCC250.11558555642886864No Hit
ACATGGGACTGCTTCTCATATGCAGGCAGCTACACCCATTGGGTGTTCGG250.11558555642886864No Hit
ACATGGGAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCA240.11096213417171391No Hit
ACATGGGATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGC240.11096213417171391No Hit
GCATGGGAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAACA240.11096213417171391No Hit
GCATGGGAGCTCTTTAAATTGGTATCAGCAGAAACCAGGGAAGGCCCCTA230.10633871191455917No Hit
GCATGGGGCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGC230.10633871191455917No Hit
GCATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTG230.10633871191455917No Hit
GCATGGGGAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTC230.10633871191455917No Hit
GCATGGGAGGAGTCAGTCTCAGTCAGGACACAGCATGGACATGAGGGTCC220.10171528965740441No Hit
GCATGGGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCAT220.10171528965740441No Hit
GCATGGGTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCT220.10171528965740441No Hit
ACATGGGGGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAG220.10171528965740441No Hit
GCATGGGAGCGGCGGTGGGTCTGGGACAGAATTCACTCTCACAATCAGCA220.10171528965740441No Hit
GCATGGGGATCAGTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCG220.10171528965740441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)