FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002047716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254094
Sequences flagged as poor quality0
Sequence length78-265
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGGGGGACCCAGACCCTGGAGCCTCAGTTGGAAGCAGCCGATCCA2932711.541791620423938No Hit
GCATGGGAGAAAGGAGACAGTCTCTTAGGGCAGAGATGTGTCTGTCCCTG235129.253268475446093No Hit
GCATGGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT132395.210276511842074No Hit
GCATGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTTT123204.848599337253143No Hit
ACATGGGAGAAAGGAGACAGTCTCTTAGGGCAGAGATGTGTCTGTCCCTG112794.438908435460892No Hit
ACATGGGGTTTTGGTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACT112564.429856667217644No Hit
GCATGGGGAGTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG70272.7655119758829407No Hit
GCATGGGGGTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGAC68672.702543153321212No Hit
ACATGGGGGGGACCCAGACCCTGGAGCCTCAGTTGGAAGCAGCCGATCCA68502.695852715924028No Hit
GCATGGGGTTTTGGTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACT64992.557714861429235No Hit
ACATGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTTT60392.3766794965642637No Hit
ACATGGGGGTCTGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGAC52292.05789983234551No Hit
ACATGGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT42451.670641573590876No Hit
GCATGGGTCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCA29121.1460325706234702No Hit
ACATGGGGAGTGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG26631.048037340511779No Hit
GCATGGGTACTGTCAGCAGTATGGTAGCTCACCTCCCCCATTGTACACTT18670.7347674482671768No Hit
GCATGGGGAGGACAGTCCTACAGGATGAGCAGGGGACCCACAGTTCACGG17680.6958054893071068No Hit
GCATGGGTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGG17020.6698308500003936No Hit
GCATGGGGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCA16590.6529079789369289No Hit
ACATGGGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCATC15160.5966295937723834No Hit
GCATGGGCAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT14860.5848229395420592No Hit
ACATGGGTCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCA14680.5777389470038646No Hit
GCATGGGGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA14240.5604225207993893No Hit
ACATGGGTACTGTCAGCAGTATGGTAGCTCACCTCCCCCATTGTACACTT13560.5336607712106544No Hit
GCATGGGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCATC12240.4817114925972278No Hit
GCATGGGTAGCTCACCTCCCCCATTGTACACTTTTGGCCAGGGGACCAAA11360.44707864018827675No Hit
GCATGGGATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA10910.4293686588427905No Hit
ACATGGGATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA10370.4081166812282069No Hit
GCATGGGGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAG9580.37702582508835314No Hit
ACATGGGTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGG9350.3679740568451046No Hit
GCATGGGTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA9100.3581351783198344No Hit
GCATGGGAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCA8740.3439671932434453No Hit
GCATGGGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTA8640.3400316418333373No Hit
GCATGGGAGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACC8240.32428943619290496No Hit
ACATGGGGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTA7900.3109085613985375No Hit
GCATGGGACTGTCAGCAGTATGGTAGCTCACCTCCCCCATTGTACACTTT7850.30894078569348354No Hit
GCATGGGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCA7520.2959534660401269No Hit
GCATGGGTCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTG7240.2849339220918243No Hit
GCATGGGAGGGACCCAGACCCTGGAGCCTCAGTTGGAAGCAGCCGATCCA6960.27391437814352165No Hit
ACATGGGTCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTG6100.24006863601659226No Hit
GCATGGGTGGGACCCAGACCCTGGAGCCTCAGTTGGAAGCAGCCGATCCA5950.2341653089014302No Hit
ACATGGGGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCA5940.2337717537604194No Hit
GCATGGGGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCA5840.2298362023503113No Hit
GCATGGGGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCTC5720.2251135406581816No Hit
ACATGGGGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCTC5530.21763599297897626No Hit
ACATGGGTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA5520.21724243783796549No Hit
GCATGGGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGG5480.21566821727392227No Hit
GCATGGGGACCAGATTCTTTCAAAAAGTCAGATGAGTAAGGGATAGAAAA4950.19480979480034946No Hit
GCATGGTAGCTCACCTCCCCCATTGTACACTTTTGGCCAGGGGACCAAAC4900.19284201909529544No Hit
GCATGGGTGGTGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGT4850.19087424339024142No Hit
GCATGGGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT4820.18969357796720898No Hit
GCATGGGGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCC4640.18260958542901445No Hit
ACATGGGAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCA4580.18024825458294963No Hit
ACATGGGACTGTCAGCAGTATGGTAGCTCACCTCCCCCATTGTACACTTT4580.18024825458294963No Hit
GCATGGGACTGTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA4430.1743449274677875No Hit
GCATGGGGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGG4260.1676544900706038No Hit
ACATGGGAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCA4060.15978338725038765No Hit
GCATGGGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTG4000.1574220564043228No Hit
ACATGGGCAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT3990.157028501263312No Hit
GCATGGGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAG3960.15584783584027959No Hit
ACATGGGGAGGACAGTCCTACAGGATGAGCAGGGGACCCACAGTTCACGG3930.15466717041724715No Hit
GCATGGGGACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATTTTG3910.15388006013522554No Hit
ACATGGGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGG3890.15309294985320393No Hit
GCATGGGGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCAT3830.1507316190071391No Hit
ACATGGGACTGTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA3700.1456154021739986No Hit
ACATGGGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTA3690.1452218470329878No Hit
GCATGGGTATGGTGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCA3620.14246696104591214No Hit
GCATGGGGTAGCTCACCTCCCCCATTGTACACTTTTGGCCAGGGGACCAA3510.13813785449479327No Hit
ACATGGGTAGCTCACCTCCCCCATTGTACACTTTTGGCCAGGGGACCAAA3490.13735074421277166No Hit
GCATGGGAGAAGAAGTGAAGTCAAGATGAAGAACCATTTGCTTTTCTGGG3470.13656363393075005No Hit
GCATGGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTC3340.13144741709760954No Hit
ACATGGGTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCT3280.12908608625154472No Hit
GCATGGGAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGT3260.1282989759695231No Hit
GCATGGGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAC3260.1282989759695231No Hit
ACATGGGAGAAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACC3260.1282989759695231No Hit
ACATGGGGACTTCACTCTCATCATCAGCAGACTGGAGCCTGAAGATTTTG3190.12554408998244745No Hit
ACATGGGGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCAT3140.1235763142773934No Hit
ACATGGGGACCAGATTCTTTCAAAAAGTCAGATGAGTAAGGGATAGAAAA3120.12278920399537178No Hit
GCATGGGAGACCAAGTTGTGCTGAGCCCAGCAAGGGAAGGTCCCCAAACA3060.12042787314930695No Hit
ACATGGGGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGG3050.12003431800829613No Hit
ACATGGGGTTTTGGTCCGAGCCTCAGTCACTGTGTTATGTCTTCGGAACT3010.11846009744425291No Hit
ACATGGGGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAG2930.11531165631616645No Hit
GCATGGGGGGTCTGGGACAGACTTCACTCTCATCATCAGCAGACTGGAGC2920.11491810117515565No Hit
GCATGGGTCCCCCATTGTACACTTTTGGCCAGGGGACCAAACTGGAGATC2910.11452454603414483No Hit
GCATGGGGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCAC2840.11176966004706919No Hit
GCATGGGCATCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTAC2790.10980188434201515No Hit
GCATGGGGAGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCC2770.10901477405999355No Hit
GCATGGGAGAAAGGAGTCAGTCTCTTAGGGCAGAGATGTGTCTGTCCCTG2760.10862121891898274No Hit
ACATGGGTATGGTGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCA2730.10744055349595032No Hit
GCATGGGAGCAGTATGGTAGCTCACCTCCCCCATTGTACACTTTTGGCCA2720.1070469983549395No Hit
GCATGGGCACTCTCATCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTG2720.1070469983549395No Hit
ACATGGGTCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGGCAGTG2720.1070469983549395No Hit
ACATGGGTGGTGCATCCAGCAGGGCCGCTGGCATCCCAGACAGGTTCAGT2710.10665344321392871No Hit
GCATGGGGTGGCAGTGGGTCTGGGACAGACTTCACTCTCATCATCAGCAG2680.10547277779089628No Hit
GCATGGGGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGT2620.10311144694483144No Hit
ACATGGGGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCA2620.10311144694483144No Hit
GCATGGGTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAG2600.10232433666280982No Hit
GCATGGGGGAGCTGCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCA2550.1003565609577558No Hit
GCATGGGGACTGTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAA2550.1003565609577558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)