FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002047718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772750
Sequences flagged as poor quality0
Sequence length66-288
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAA34767844.992300226463925No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAT17590022.762859915884828No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAAATATCAAAAG15493720.050080879974118No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG191272.475186023940472No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAC87171.128049175024264No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAGAGC74100.9589129731478485No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAT72510.9383371077321255No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG69550.9000323519896474No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGAGGA36200.46845681009382073No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGTAGCCACAGTTCCTGGGAA23710.3068262698155937No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGT17500.2264639275315432No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG13680.17703008735037207No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAAATACCAGCTG13460.17418311226140407No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGTAGCCACAGTTCGTCTGAT12470.1613717243610482No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTGGGAA11730.15179553542542865No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAG10490.13574894856033645No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAC10010.1295373665480427No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAAT8980.116208346813329No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACGGTTCCTGGGAA8330.10779682950501457No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGTAGACCTGGG7890.10210287932707861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)