FastQCFastQC Report
Wed 8 May 2024
EGAF00002047723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141149
Total Bases30.3 Mbp
Sequences flagged as poor quality0
Sequence length68-288
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG8976863.59804178563079No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG2656818.822662576426332No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTTG56243.9844419726671814No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG33372.364168361093596No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTTG31782.251521441880566No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA10790.7644404140305635No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG10570.7488540478501442No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACCTAG9080.6432918405373045No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGTAGCCTTGGGCTGACCTAG6980.49451289063330245No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAAATATCAAAAG5000.35423559500952895No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGTCTTGGGCTGACCTAG3830.27134446577729915No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGT3730.2642597538771086No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA3580.2536326860268227No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCTAG3480.24654797412663212No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGACTGACCTAG3470.2458395029366131No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACCTAG3100.21962606890590797No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAA2830.20049734677539338No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGTAGCCTTGGGCTGACCTAG2500.17711779750476447No Hit
CTTACCTGAGGAGACGGTGACCTTTGTCCCTTGGCCCCAGATGTCAAAAG2380.16861614322453577No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAT2370.16790767203451673No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAACCTTGGGCTGACCTAG2170.15373824823413557No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATGGTCAGAGC1750.12398245825333512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)