FastQCFastQC Report
Wed 8 May 2024
EGAF00002047724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184624
Total Bases38.6 Mbp
Sequences flagged as poor quality0
Sequence length80-288
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAG4500524.3765707600312No Hit
CTTACCTGAGGAGACGGTGACCGTGGTCCCTTTGCCCCAGACGTCCATGT3722920.164767310858828No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAG2576813.957015339284167No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAT167469.07032671808649No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAAGAG123606.6946875812462086No Hit
CTTACCTGAGGAGACGGTGACCAGGGTACCCTGGCCCCAGTAGTCGAAAG77094.175513476037785No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG62723.397174798509403No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG41352.2396871479330964No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA32411.7554597452118899No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG32301.749501689921137No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG20591.1152396221509664No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGGAGTCAAGGA16140.8742092035704999No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGAGGA10240.5546407834301066No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTATATTCAAAAAC9760.5286419967068203No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAGC9360.5069763411040819No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTATATTCAAAAAC8590.46526995406881017No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAGAG7170.3883568766790883No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCCTGGGAA6790.3677745038564867No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTCAAT6120.33148453072189965No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGC5910.3201100615304619No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA5420.29356963341710723No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACCGAG4890.2648626397434786No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGC4330.2345307218996447No Hit
CTTACCTGAGGAGACGGTGACCAGGGTACCCTGGCCCCAATAGTCAAGAG4300.23290579772943928No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATAGTCAATTC4220.22857266660889158No Hit
CTTACCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAATAGTCAAGAG3680.19932403154519457No Hit
TCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTCTGAT3550.19228269347430454No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACCGAG3480.18849120374382528No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA2510.13595198890718432No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGAAGTCCCCCG2450.13270214056677357No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACCGAG2080.11266140913424041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)