FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002047725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141149
Sequences flagged as poor quality0
Sequence length45-265
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGTGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCTGATTATTA135599.606160865468407No Hit
ACATGGGTGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCTGATTATTA81795.794585863165874No Hit
GCATGGGTGAGTCACCATCTCCTGCACTGGAACCAGCGGTGGTGTTGGGA49553.510474746544432No Hit
ACATGGGTATCGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCC38872.753827515604078No Hit
GCATGGGTATCGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCC38162.7035260611127248No Hit
ACATGGGTGAGTCACCATCTCCTGCACTGGAACCAGCGGTGGTGTTGGGA29512.0906984817462395No Hit
GCATGGGGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCTGATTATTAC25771.825730256679112No Hit
GCATGGGTGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGGTG23951.6967885000956435No Hit
GCATGGGTCTGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAGGCTG23051.6330260929939284No Hit
GCATGGGACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGGCGG21961.5558027332818511No Hit
GCATGGGAGGATGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCT15781.1179675378500733No Hit
ACATGGGTCTGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAGGCTG15531.1002557580995969No Hit
ACATGGGTGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGGTG14761.0457034764681294No Hit
GCATGGGCCTGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCTGATTAT14561.0315340526677481No Hit
ACATGGGACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGGCGG14521.0287001679076722No Hit
ACATGGGGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCTGATTATTAC14311.0138222729172717No Hit
GCATGGGTCGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCCGG14131.0010697914969289No Hit
GCATGGGGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCAT12950.91747019107468No Hit
GCATGGGTATGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGG12690.8990499401341844No Hit
GCATGGGTGATGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCCAAA11130.7885284344912113No Hit
GCATGGGGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGGTGA10990.7786098378309446No Hit
GCATGGGGAGGATGAGGCTGATTATTACTGCCAGTCATATGACGGCAACC10630.7531048749902586No Hit
GCATGGGAGCCTCCCTGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCT9630.6822577559883527No Hit
GCATGGGATGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGG9600.6801323424182956No Hit
GCATGGGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGAGT9380.6645459762378763No Hit
GCATGGGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT9270.6567527931476667No Hit
ACATGGGTGATGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCCAAA8780.6220377048367328No Hit
GCATGGGGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGAG8620.6107021657964279No Hit
ACATGGGTTATGATGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCC8260.5851972029557417No Hit
GCATGGGTATGATGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCCA8200.5809463758156275No Hit
GCATGGGAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCATATGACGG8040.5696108367753225No Hit
ACATGGGTATGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGG7580.5370211620344458No Hit
GCATGGGGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATCATTGGGC7330.5193093822839694No Hit
GCATGGGGATGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGG7240.5129331415737979No Hit
GCATGGGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGGTGAC7220.5115161991937598No Hit
GCATGGGTTATGATGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCC7040.4987637177734167No Hit
ACATGGGTTATTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTC6930.4909705346832071No Hit
ACATGGGAGGATGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCT6670.4725502837427116No Hit
ACATGGGGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCAT6560.464757100652502No Hit
ACATGGGTCTCCTGCACTGGGAGCAGCTCCAATATCGGGGCAAATTATGA6550.4640486294624829No Hit
ACATGGGTCGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCCGG6140.4350013106717015No Hit
GCATGGGATCGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCCG5930.4201234156813013No Hit
GCATGGGATGATGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCCAA5920.4194149444912823No Hit
ACATGGGATGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGG5890.41728953092122506No Hit
GCATGGGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAGGCTGAGGA5860.4151641173511679No Hit
ACATGGGAGCCTCCCTGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCT5830.41303870378111074No Hit
ACATGGGTATGATGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCCA5730.40595399188092013No Hit
GCATGGGCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCATATGAC5520.3910760968905199No Hit
GCATGGGTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTA5520.3910760968905199No Hit
GCATGGGTGCTAAAAACATTCGCCCCTCAGGGGTCCCTGACCGATTCTCT5300.37548973071010067No Hit
GCATGGGGAGGACAGTCCTACAGGATGAGCAGGGGACCCACAGTTCACGG5220.3698219611899482No Hit
GCATGGGTCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCT5040.3570694797696052No Hit
GCATGGGTCTCCTGCACTGGGAGCAGCTCCAATATCGGGGCAAATTATGA5030.3563610085795861No Hit
GCATGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGAACCTCAGCCTC5020.35565253738956704No Hit
GCATGGGTCCTGCACTGGGAGCAGCTCCAATATCGGGGCAAATTATGATG4870.34502546953928115No Hit
ACATGGGCCTGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCTGATTAT4850.34360852715924306No Hit
ACATGGGGCCATCATTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT4820.3414831135891859No Hit
GCATGGGGGTGTCACAGTGTGTGGTATTCGGCGGAGGGACCAAGCTGACC4730.33510687287901436No Hit
GCATGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTCGTTTTCG4620.3273136897888047No Hit
GCATGGGTTATTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTC4600.3258967474087666No Hit
ACATGGGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAGGCTGAGGA4530.3209374490786332No Hit
ACATGGGGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGGTGA4440.3145612083684617No Hit
ACATGGGTCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCT4400.31172732360838545No Hit
GCATGGGTGGGAGTCGTTTTCGGCGGAGGGACCACGGTGACCGTCCTAGG4400.31172732360838545No Hit
ACATGGGTCCTGCACTGGGAGCAGCTCCAATATCGGGGCAAATTATGATG4310.30535108289821394No Hit
ACATGGGATCGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCCG4260.30180872694811867No Hit
GCATGGGCTCTGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATCATT4110.2911816590978328No Hit
GCATGGGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCCGGAAC4110.2911816590978328No Hit
ACATGGGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGAGT4070.28834777433775655No Hit
GCATGGGTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCATATGA4040.2862223607676994No Hit
ACATGGGTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTA3920.2777207064874707No Hit
ACATGGGGATGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGG3910.2770122352974516No Hit
GCATGGGTCTGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATCATTG3850.2727614081573373No Hit
ACATGGGAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCATATGACGG3810.26992752339726106No Hit
GCATGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTTT3760.2663851674471657No Hit
ACATGGGATTATTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTT3740.26496822506712764No Hit
GCATGGGAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGT3680.26071739792701326No Hit
ACATGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGAACCTCAGCCTC3630.25717504197691804No Hit
GCATGGGAAGCAGTGGTATTAACGCAGAGTACATGGGAGGCTGAGGATGA3630.25717504197691804Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCATGGGCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGA3620.25646657078689894No Hit
GCATGGGGAGGCTTGGGTCTCCCCACACACTGCCTGCCAGGACAGTCCTA3600.25504962840686085No Hit
GCATGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGAC3590.2543411572168418No Hit
ACATGGGGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATCATTGGGC3570.25292421483680366No Hit
GCATGGGGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGAGTC3480.24654797412663212No Hit
ACATGGGATGATGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCCAA3410.24158867579649876No Hit
GCATGGGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATCATTGGGCT3380.23946326222644157No Hit
GCATGGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT3380.23946326222644157No Hit
ACATGGGATTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGG3260.23096160794621287No Hit
GCATGGGTGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGA3230.22883619437615568No Hit
GCATGGGGCTGAGGATGAGGCTGATTATTACTGCCAGTCATATGACGGCA3210.22741925199611757No Hit
GCATGGGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGAGTCG3210.22741925199611757No Hit
GCATGGGCTGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAGGCTGA3190.22600230961607945No Hit
ACATGGGTGCTAAAAACATTCGCCCCTCAGGGGTCCCTGACCGATTCTCT3190.22600230961607945No Hit
GCATGGGCATATGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCAC3050.21608371295581263No Hit
GCATGGGGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCCGGAA3040.21537524176579362No Hit
GCATGGGCCGATTCTCTGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCC3040.21537524176579362No Hit
ACATGGGGAGGATGAGGCTGATTATTACTGCCAGTCATATGACGGCAACC3010.21324982819573643No Hit
GCATGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTCTGGAACC2990.2118328858156983No Hit
ACATGGGTCTGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATCATTG2990.2118328858156983No Hit
ACATGGGAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTCACCGT2980.21112441462567927No Hit
ACATGGGGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGAG2760.19553804844525996No Hit
GCATGGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAGGCTGAGGAT2720.19270416368518373No Hit
GCATGGGATTATTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTT2700.1912872213051456No Hit
GCATGGGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGGTGACCGT2640.18703639416503126No Hit
GCATGGGGAAGTCTGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAG2630.18632792297501222No Hit
ACATGGGATTCTCTGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATC2610.1849109805949741No Hit
GCATGGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGAACCTCAGCC2570.18207709583489787No Hit
ACATGGGTTCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTC2560.18136862464487882No Hit
GCATGGGATTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGG2540.1799516822648407No Hit
GCATGGGCAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTT2530.17924321107482163No Hit
ACATGGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAGGCTGAGGAT2520.1785347398848026No Hit
ACATGGGCTCTGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATCATT2480.17570085512472636No Hit
ACATGGGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGGTGAC2450.1735754415546692No Hit
GCATGGGTATGCTAAAAACATTCGCCCCTCAGGGGTCCCTGACCGATTCT2430.17215849917463108No Hit
GCATGGGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGA2430.17215849917463108No Hit
GCATGGGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCATA2400.1700330856045739No Hit
ACATGGGTATTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCG2380.16861614322453577No Hit
ACATGGGTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGGCG2370.16790767203451673No Hit
ACATGGGGGTGTCACAGTGTGTGGTATTCGGCGGAGGGACCAAGCTGACC2370.16790767203451673No Hit
ACATGGGTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCATATGA2370.16790767203451673No Hit
GCATGGGTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGGCG2350.16649072965447861No Hit
ACATGGGTGGGAGTCGTTTTCGGCGGAGGGACCACGGTGACCGTCCTAGG2310.16365684489440235No Hit
ACATGGGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATCATTGGGCT2300.1629483737043833No Hit
GCATGGGGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCACGGTGACCG2130.15090436347405933No Hit
GCATGGGGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAGGCTGAGG2130.15090436347405933No Hit
ACATGGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGAACCTCAGCC2110.14948742109402122No Hit
ACATGGGTGAGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGA2070.14665353633394498No Hit
GCATGGGGATGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCCAAAC2060.1459450651439259No Hit
GCATGGGGTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGGC2060.1459450651439259No Hit
GCATGGGTTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTC2040.14452812276388782No Hit
GCATGGGCGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCCGGA2000.1416942380038116No Hit
ACATGGGTTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTC1970.1395688244337544No Hit
ACATGGGGTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGGC1970.1395688244337544No Hit
GCATGGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGAGTCGT1950.13815188205371629No Hit
GCATGGGCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGAAC1940.13744341086369724No Hit
GCATGGGGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGG1920.13602646848365912No Hit
GCATGGGTATCAACACTTTCCCGGAACAGCCCCCAAACTCCTCATCTATG1910.13531799729364005No Hit
ACATGGGGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGAGTC1890.13390105491360194No Hit
GCATGGGTATTACTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCG1880.13319258372358286No Hit
ACATGGGAGCCCCCAGAGAGCAGACCCCAGGTGCTGGCCCCGGGGGTTTT1830.1296502277734876No Hit
GCATGGGTGCCAGTCATATGACGGCAACCTGGGAGTCGTTTTCGGCGGAG1820.12894175658346851No Hit
GCATGGGATGCTAAAAACATTCGCCCCTCAGGGGTCCCTGACCGATTCTC1810.12823328539344947No Hit
ACATGGGTATGCTAAAAACATTCGCCCCTCAGGGGTCCCTGACCGATTCT1810.12823328539344947No Hit
GCATGGGTTCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTC1800.12752481420343043No Hit
GCATGGGAGCTCCAATATCGGGGCAAATTATGATGTGCAGTGGTATCAAC1780.1261078718233923No Hit
ACATGGGGGGCAAATTATGATGTGCAGTGGTATCAACACTTTCCCGGAAC1730.122565515873297No Hit
GCATGGGAGCCCCCAAACTCCTCATCTATGCTAAAAACATTCGCCCCTCA1730.122565515873297No Hit
ACATGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGAC1720.12185704468327796No Hit
GCATGGGGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAGGTC1710.1211485734932589No Hit
GCATGGGAGGCACAGAGCTGTCTGCTCTCCACAGGGCATGAGTGGAAAGG1700.12044010230323984No Hit
GCATGGGGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTG1700.12044010230323984No Hit
ACATGGGGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTCGTTTTCG1680.11902315992320173No Hit
GCATGGGTGCACTGGGAGCAGCTCCAATATCGGGGCAAATTATGATGTGC1670.11831468873318267No Hit
ACATGGGCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCATATGAC1660.11760621754316361No Hit
GCATGGGGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGAACCTCAG1570.11122997683299209No Hit
ACATGGGGCTGATTATTACTGCCAGTCATATGACGGCAACCTGGGAGTCG1560.11052150564297303No Hit
GCATGGGGTGGTCGGGCTTGTGGAGACAGGAAACATCTCAGAGCCTCAGA1550.10981303445295398No Hit
GCATGGGTGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCCAAACTC1540.10910456326293493No Hit
GCATGGGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGAACCTCAGC1520.10768762088289681No Hit
ACATGGGCATATGACGGCAACCTGGGAGTCGTTTTCGGCGGAGGGACCAC1520.10768762088289681No Hit
ACATGGGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCATA1520.10768762088289681No Hit
ACATGGGGGAACCTCAGCCTCCCTGGCCATCATTGGGCTCCAGGCTGAGG1500.10627067850285869No Hit
GCATGGGATTCTCTGGCTCCAAGTCTGGAACCTCAGCCTCCCTGGCCATC1480.10485373612282058No Hit
GCATGGGCAGCCCCCAAACTCCTCATCTATGCTAAAAACATTCGCCCCTC1450.10272832255276339No Hit
GCATGGGGTGCAGTGGTATCAACACTTTCCCGGAACAGCCCCCAAACTCC1450.10272832255276339No Hit
GCATGGGCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTG1440.10201985136274433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)