FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002047726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217016
Sequences flagged as poor quality0
Sequence length50-265
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGGAGGCTTGGGTCTCCCCACACACTGCCTGCCAGGACAGTCCTA5148223.722674825819297No Hit
ACATGGGGAGGCTTGGGTCTCCCCACACACTGCCTGCCAGGACAGTCCTA139006.405057691598776No Hit
GCATGGGATCACACAACAGCCACATCCCTCCCCTACAGAAGCCCCCAGAG57632.6555645666678953No Hit
GCATGGGGGAGGTGAGCAGCCTCAGATCTGAAGACACGGCCATCTATTAT57422.64588786080289No Hit
GCATGGGTGCCGAGAAATTCCAGGGCACAGTCACCATAACCGCGGACACG47622.1943082537693073No Hit
GCATGGGTCTGAAGACACGGCCATCTATTATTGTGCAACGGGTCTGGAGG38971.7957201312345634No Hit
ACATGGGATCACACAACAGCCACATCCCTCCCCTACAGAAGCCCCCAGAG35241.623843403251373No Hit
ACATGGGGGAGGTGAGCAGCCTCAGATCTGAAGACACGGCCATCTATTAT30551.4077303055995871No Hit
GCATGGGTCAACAGACACGACCTATCTGGAGGTGAGCAGCCTCAGATCTG30201.3916024624912449No Hit
GCATGGGTAACCGCGGACACGTCAACAGACACGACCTATCTGGAGGTGAG28621.3187967707450141No Hit
ACATGGGTCTGAAGACACGGCCATCTATTATTGTGCAACGGGTCTGGAGG28241.3012865410845282No Hit
ACATGGGTGCCGAGAAATTCCAGGGCACAGTCACCATAACCGCGGACACG26771.233549600029491No Hit
GCATGGGAACCGCGGACACGTCAACAGACACGACCTATCTGGAGGTGAGC26681.22940244037306No Hit
GCATGGGGAAATTCCAGGGCACAGTCACCATAACCGCGGACACGTCAACA26181.2063626645039995No Hit
GCATGGGAGCACTGAACACAGAGAAGTCCCCTTGGACGTTGGACTGACCT25661.182401297600177No Hit
ACATGGGTAACCGCGGACACGTCAACAGACACGACCTATCTGGAGGTGAG24801.1427728831053932No Hit
GCATGGGATGGTGAGACGGTCTATGCCGAGAAATTCCAGGGCACAGTCAC22161.0211228665167544No Hit
ACATGGGTATCTGGAGGTGAGCAGCCTCAGATCTGAAGACACGGCCATCT21480.9897887713348325No Hit
ACATGGGTCAACAGACACGACCTATCTGGAGGTGAGCAGCCTCAGATCTG21330.9828768385741143No Hit
GCATGGGAACAGACACGACCTATCTGGAGGTGAGCAGCCTCAGATCTGAA20320.9363364913186125No Hit
GCATGGGCAGCCTCAGATCTGAAGACACGGCCATCTATTATTGTGCAACG19540.9003944409628784No Hit
GCATGGGAGACACGACCTATCTGGAGGTGAGCAGCCTCAGATCTGAAGAC19250.8870313709588233No Hit
ACATGGGAACCGCGGACACGTCAACAGACACGACCTATCTGGAGGTGAGC18720.8626092085376194No Hit
ACATGGGGAAATTCCAGGGCACAGTCACCATAACCGCGGACACGTCAACA16260.7492535112618425No Hit
ACATGGGATGGTGAGACGGTCTATGCCGAGAAATTCCAGGGCACAGTCAC14930.687967707450142No Hit
GCATGGGGGGCGGAGCAGGAAGGAAACCGCTCCCGAGCACGGCGGCGGCG14750.6796733881372802No Hit
GCATGGGTCTGGAGGTGAGCAGCCTCAGATCTGAAGACACGGCCATCTAT14750.6796733881372802No Hit
ACATGGGTCTGGAGGTGAGCAGCCTCAGATCTGAAGACACGGCCATCTAT14660.6755262284808493No Hit
GCATGGGTATCTGGAGGTGAGCAGCCTCAGATCTGAAGACACGGCCATCT14540.6699966822722748No Hit
GCATGGGAGCCTCAGATCTGAAGACACGGCCATCTATTATTGTGCAACGG13850.6382017915729715No Hit
GCATGGGACGGCCATCTATTATTGTGCAACGGGTCTGGAGGCCAGCAGTG13780.6349762229513031No Hit
GCATGGGTCAGATCTGAAGACACGGCCATCTATTATTGTGCAACGGGTCT13560.6248387215689165No Hit
GCATGGGGACACGTCAACAGACACGACCTATCTGGAGGTGAGCAGCCTCA13280.6119364470822427No Hit
ACATGGGAGCACTGAACACAGAGAAGTCCCCTTGGACGTTGGACTGACCT13060.6017989456998561No Hit
GCATGGGGGAGGTGAGCAACCTCAGATCTGAAGACACGGCCATCTATTAT11970.5515722343053047No Hit
ACATGGGAGACACGACCTATCTGGAGGTGAGCAGCCTCAGATCTGAAGAC11850.5460426880967302No Hit
GCATGGGTGAGCAGCCTCAGATCTGAAGACACGGCCATCTATTATTGTGC11770.5423563239576805No Hit
GCATGGGGGCACAGTCACCATAACCGCGGACACGTCAACAGACACGACCT11630.5359051867143436No Hit
GCATGGGGACACGGCCATCTATTATTGTGCAACGGGTCTGGAGGCCAGCA10790.49719836325432226No Hit
GCATGGGGGGCACAGTCACCATAACCGCGGACACGTCAACAGACACGACC10430.4806097246285988No Hit
ACATGGGAGCCTCAGATCTGAAGACACGGCCATCTATTATTGTGCAACGG10190.4695506322114499No Hit
GCATGGGCCTCAGATCTGAAGACACGGCCATCTATTATTGTGCAACGGGT10180.4690898366940687No Hit
GCATGGGTCACCATAACCGCGGACACGTCAACAGACACGACCTATCTGGA9990.46033472186382574No Hit
GCATGGGTGGTGAGACGGTCTATGCCGAGAAATTCCAGGGCACAGTCACC9920.45710915324215723No Hit
ACATGGGTCAGATCTGAAGACACGGCCATCTATTATTGTGCAACGGGTCT9860.45434438013787No Hit
ACATGGGAACAGACACGACCTATCTGGAGGTGAGCAGCCTCAGATCTGAA9640.4442068787554834No Hit
GCATGGGAGGGCTTGACTGGATGGGACTTGTTAATCCTGAAAATGGTGAG9520.438677332546909No Hit
GCATGGGGACCTATCTGGAGGTGAGCAGCCTCAGATCTGAAGACACGGCC9390.43268699082095335No Hit
ACATGGGGACACGTCAACAGACACGACCTATCTGGAGGTGAGCAGCCTCA9370.4317653997861909No Hit
ACATGGGTGAGCAGCCTCAGATCTGAAGACACGGCCATCTATTATTGTGC9220.4248534670254728No Hit
GCATGGGGATCACACAACAGCCACATCCCTCCCCTACAGAAGCCCCCAGA8840.4073432373649869No Hit
ACATGGGGACCTATCTGGAGGTGAGCAGCCTCAGATCTGAAGACACGGCC8540.39351937184355074No Hit
GCATGGGGGGCTTGACTGGATGGGACTTGTTAATCCTGAAAATGGTGAGA8280.38153868839163935No Hit
ACATGGGACGGCCATCTATTATTGTGCAACGGGTCTGGAGGCCAGCAGTG7880.36310686769639106No Hit
ACATGGGCCTCAGATCTGAAGACACGGCCATCTATTATTGTGCAACGGGT7810.3598812990747226No Hit
ACATGGGTCACCATAACCGCGGACACGTCAACAGACACGACCTATCTGGA7780.358498912522579No Hit
ACATGGGCAGCCTCAGATCTGAAGACACGGCCATCTATTATTGTGCAACG7720.35573413941829174No Hit
GCATGGGGAGGACAGTCCTACAGGATGAGCAGGGGACCCACAGTTCACGG7300.33638072768828103No Hit
ACATGGGGACACGGCCATCTATTATTGTGCAACGGGTCTGGAGGCCAGCA7240.3336159545839938No Hit
GCATGGGTGGGTGCAACACGCCCCTGGAAAAGGGCTTGACTGGATGGGAC6430.29629151767611606No Hit
ACATGGGGGAGGTGAGCAACCTCAGATCTGAAGACACGGCCATCTATTAT6330.291683562502304No Hit
ACATGGGTGGTGAGACGGTCTATGCCGAGAAATTCCAGGGCACAGTCACC6200.2856932207763483No Hit
ACATGGGGGGCTTGACTGGATGGGACTTGTTAATCCTGAAAATGGTGAGA5700.2626534449072879No Hit
ACATGGGGGCACAGTCACCATAACCGCGGACACGTCAACAGACACGACCT5650.2603494673203819No Hit
ACATGGGTGGGTGCAACACGCCCCTGGAAAAGGGCTTGACTGGATGGGAC5600.2580454897334759No Hit
ACATGGGAGGGCTTGACTGGATGGGACTTGTTAATCCTGAAAATGGTGAG5530.2548199211118074No Hit
GCATGGGAGCATCACACAACAGCCACATCCCTCCCCTACAGAAGCCCCCA5260.24237844214251483No Hit
GCATGGGACTGGATGGGACTTGTTAATCCTGAAAATGGTGAGACGGTCTA5150.23730969145132155No Hit
ACATGGGGGGCGGAGCAGGAAGGAAACCGCTCCCGAGCACGGCGGCGGCG5120.23592730489917793No Hit
GCATGGGGTCACCATAACCGCGGACACGTCAACAGACACGACCTATCTGG5120.23592730489917793No Hit
GCATGGGTACATTCACTGGGTGCAACACGCCCCTGGAAAAGGGCTTGACT5090.23454491834703434No Hit
ACATGGGGGGCACAGTCACCATAACCGCGGACACGTCAACAGACACGACC5040.23224094076012827No Hit
GCATGGGGACTGGATGGGACTTGTTAATCCTGAAAATGGTGAGACGGTCT4990.22993696317322224No Hit
ACATGGGTACATTCACTGGGTGCAACACGCCCCTGGAAAAGGGCTTGACT4990.22993696317322224No Hit
ACATGGGCTTGTTAATCCTGAAAATGGTGAGACGGTCTATGCCGAGAAAT4900.2257898035167914No Hit
ACATGGGGTTAATCCTGAAAATGGTGAGACGGTCTATGCCGAGAAATTCC4880.22486821248202896No Hit
ACATGGGGATCACACAACAGCCACATCCCTCCCCTACAGAAGCCCCCAGA4630.21334832454749877No Hit
GCATGGGGGCCATCTATTATTGTGCAACGGGTCTGGAGGCCAGCAGTGGC4520.20827957385630555No Hit
GCATGGGGATACATTCACTGGGTGCAACACGCCCCTGGAAAAGGGCTTGA4420.20367161868249345No Hit
ACATGGGACTGGATGGGACTTGTTAATCCTGAAAATGGTGAGACGGTCTA4370.2013676410955874No Hit
GCATGGGGTTAATCCTGAAAATGGTGAGACGGTCTATGCCGAGAAATTCC4280.19722048143915655No Hit
ACATGGGGTCACCATAACCGCGGACACGTCAACAGACACGACCTATCTGG4250.19583809488701293No Hit
GCATGGGCTTGTTAATCCTGAAAATGGTGAGACGGTCTATGCCGAGAAAT4110.18938695764367602No Hit
ACATGGGGACTGGATGGGACTTGTTAATCCTGAAAATGGTGAGACGGTCT4070.18754377557415122No Hit
ACATGGGAGTCTGAGGCTGAGGTGAGGAAGCCTGGGGCTACAGTTAAAAT3930.18109263833081432No Hit
GCATGGGGGTGAGACGGTCTATGCCGAGAAATTCCAGGGCACAGTCACCA3800.17510229660485863No Hit
GCATGGGAAGACTTCTGGATATATTTTCACCCACCGATACATTCACTGGG3650.16819036384414052No Hit
CCATGGGGAGGCTTGGGTCTCCCCACACACTGCCTGCCAGGACAGTCCTA3640.1677295683267593No Hit
GCATGGGAGTCTGAGGCTGAGGTGAGGAAGCCTGGGGCTACAGTTAAAAT3510.16173922660080362No Hit
ACATGGGGATACATTCACTGGGTGCAACACGCCCCTGGAAAAGGGCTTGA3470.15989604453127879No Hit
GCATGGGGGAAAAGGGCTTGACTGGATGGGACTTGTTAATCCTGAAAATG3290.15160172521841708No Hit
GCATGGGGGCTGAGGTGAGGAAGCCTGGGGCTACAGTTAAAATCTCCTGC3160.1456113834924614No Hit
GCATGGGGAGCATCACACAACAGCCACATCCCTCCCCTACAGAAGCCCCC3150.14515058797508018No Hit
GCATGGGGCCCCTGGAAAAGGGCTTGACTGGATGGGACTTGTTAATCCTG2890.1331699045231688No Hit
ACATGGGGGCCATCTATTATTGTGCAACGGGTCTGGAGGCCAGCAGTGGC2870.1322483134884064No Hit
GCATGGGATGGGACTTGTTAATCCTGAAAATGGTGAGACGGTCTATGCCG2410.11105171968887086No Hit
GCATGGGGGAGCCTCAGTCACTGTGTTATGTCTTCGGAACTGGGACCAAG2410.11105171968887086No Hit
ACATGGGGGAAAAGGGCTTGACTGGATGGGACTTGTTAATCCTGAAAATG2330.1073653555498212No Hit
GCATGGGACACACAACAGCCACATCCCTCCCCTACAGAAGCCCCCAGAGC2300.1059829689976776No Hit
ACATGGGGCCCCTGGAAAAGGGCTTGACTGGATGGGACTTGTTAATCCTG2300.1059829689976776No Hit
ACATGGGGGTGAGACGGTCTATGCCGAGAAATTCCAGGGCACAGTCACCA2180.1004534227891031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)