FastQCFastQC Report
Wed 8 May 2024
EGAF00002047732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002047732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172965
Total Bases32.1 Mbp
Sequences flagged as poor quality0
Sequence length68-288
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAG7058640.80941230884861No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG3822022.096956031567082No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAG2433114.06700777613968No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAG119436.90486514612783No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGT40382.334576359379065No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGTAGACCTGGG33871.9581996357644609No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAAAAA25981.502037984563351No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG13390.7741450582487787No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTGT11480.6637180932558611No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGT9590.5544474315612985No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA9080.524961697453242No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACGTAG8810.5093516029254473No Hit
CTTACCTGAGGAGACGGTGACCAGGGTCCCCTGGCCCCAGTAGTCAAAAA6690.3867834532998005No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGTAGCCTTGGGCTGACGTAG6300.36423553898187494No Hit
CTTACCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAATGGTCAGAGC6020.34804729280490276No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAG5850.3382187147688839No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAACCTTGGGCTGACGTAG4610.2665279102708641No Hit
GAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCAAG4170.24108923770705054No Hit
GAGGAGGGTGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACTTTA4070.23530772121527474No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGACTGACGTAG3950.22836990142514382No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGACAGCCTTGGGCTGACGTAG3590.207556442054751No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTGT3500.20235307721215276No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGTCTTGGGCTGACGTAG2640.15263203538288092No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGTTGACGTAG2570.14858497383863786No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGTAGCCTTGGGCTGACGTAG2510.1451160639435724No Hit
GAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAACCTTGGGCTGACGTAG1880.10869251004538491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)